Detection of a new HIV-1 reverse transcriptase mutation (Q145M) conferring resistance to nucleoside and non-nucleoside inhibitors in a patient failing highly active antiretroviral therapy

Stefania Paolucci, Fausto Baldanti, Marco Tinelli, Giovanni Maga, Giuseppe Gerna

Research output: Contribution to journalArticle

Abstract

The novel Q145M change was detected in HIV-1 reverse transcriptase (RT) of a patient failing highly active antiretroviral therapy (HAART). After Q145M insertion into a wild-type HIV-1 RT and the cloning of both wild-type and mutated RT sequences into HIV-1 HXB2 backbone, recombinant wild-type and mutant HIV-1 strains were obtained. Mutant showed a greater than16-fold increase in IC50 values of all RT inhibitors. Multi-drug-resistance-associated mutations Q145M and Q151M map to an RT region crucial for drug recognition and processing.

Original languageEnglish
Pages (from-to)924-927
Number of pages4
JournalAIDS (London, England)
Volume17
Issue number6
DOIs
Publication statusPublished - Apr 11 2003

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Fingerprint Dive into the research topics of 'Detection of a new HIV-1 reverse transcriptase mutation (Q145M) conferring resistance to nucleoside and non-nucleoside inhibitors in a patient failing highly active antiretroviral therapy'. Together they form a unique fingerprint.

  • Cite this