The novel Q145M change was detected in HIV-1 reverse transcriptase (RT) of a patient failing highly active antiretroviral therapy (HAART). After Q145M insertion into a wild-type HIV-1 RT and the cloning of both wild-type and mutated RT sequences into HIV-1 HXB2 backbone, recombinant wild-type and mutant HIV-1 strains were obtained. Mutant showed a greater than16-fold increase in IC50 values of all RT inhibitors. Multi-drug-resistance-associated mutations Q145M and Q151M map to an RT region crucial for drug recognition and processing.
ASJC Scopus subject areas
- Immunology and Allergy