Abstract
Background: A rising isolation trend of drug-resistant M. bovis from human clinical cases is documented in the literature. Here we assessed Mycobacterium tuberculosis complex isolates from cattle for drug susceptibility by the gold standard agar proportion method and a simplified resazurin microtitre assay (d-REMA). A total of 38 M. tuberculosis complex strains, including M. bovis (n = 36) and M. caprae (n = 2) isolates, from cattle in Tunisia were tested against isoniazid, rifampin, streptomycin, ethambutol, kanamycin and pyrazinamide. Results: M. caprae isolates were found to be susceptible to all test drugs. All M. bovis strains were resistant to pyrazinamide, as expected. In addition, one M. bovis isolate showed high-level resistance to streptomycin (MIC > 500.0 μg/ml). Concordant results with the two methods were found. The most common target genes associated with streptomycin resistance, namely the rrs, rpsL and gidB genes, were DNA sequenced. A non-synonymous mutation at codon 43 (K43R) was found in the rpsL gene. To the best of our knowledge, this is the first report describing the isolation of a streptomycin-resistant M. bovis isolate from animal origin. Conclusions: Antitubercular drug susceptibility testing of M. bovis isolates from animals should be performed in settings where bTB is endemic in order to estimate the magnitude of the risk of drug-resistant tuberculosis transmission to humans.
| Original language | English |
|---|---|
| Article number | 296 |
| Journal | BMC Veterinary Research |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 29 2018 |
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Keywords
- Bovine tuberculosis
- d-REMA
- Mycobacterium tuberculosis complex
- Resazurin microtitre assay
- Streptomycin resistance
ASJC Scopus subject areas
- veterinary(all)
Cite this
Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle 06 Biological Sciences 0604 Genetics. / Djemal, Saif Eddine; Camperio, Cristina; Armas, Federica; Siala, Mariam; Smaoui, Salma; Messadi-Akrout, Feriele; Gdoura, Radhouane; Marianelli, Cinzia.
In: BMC Veterinary Research, Vol. 14, No. 1, 296, 29.09.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle 06 Biological Sciences 0604 Genetics
AU - Djemal, Saif Eddine
AU - Camperio, Cristina
AU - Armas, Federica
AU - Siala, Mariam
AU - Smaoui, Salma
AU - Messadi-Akrout, Feriele
AU - Gdoura, Radhouane
AU - Marianelli, Cinzia
PY - 2018/9/29
Y1 - 2018/9/29
N2 - Background: A rising isolation trend of drug-resistant M. bovis from human clinical cases is documented in the literature. Here we assessed Mycobacterium tuberculosis complex isolates from cattle for drug susceptibility by the gold standard agar proportion method and a simplified resazurin microtitre assay (d-REMA). A total of 38 M. tuberculosis complex strains, including M. bovis (n = 36) and M. caprae (n = 2) isolates, from cattle in Tunisia were tested against isoniazid, rifampin, streptomycin, ethambutol, kanamycin and pyrazinamide. Results: M. caprae isolates were found to be susceptible to all test drugs. All M. bovis strains were resistant to pyrazinamide, as expected. In addition, one M. bovis isolate showed high-level resistance to streptomycin (MIC > 500.0 μg/ml). Concordant results with the two methods were found. The most common target genes associated with streptomycin resistance, namely the rrs, rpsL and gidB genes, were DNA sequenced. A non-synonymous mutation at codon 43 (K43R) was found in the rpsL gene. To the best of our knowledge, this is the first report describing the isolation of a streptomycin-resistant M. bovis isolate from animal origin. Conclusions: Antitubercular drug susceptibility testing of M. bovis isolates from animals should be performed in settings where bTB is endemic in order to estimate the magnitude of the risk of drug-resistant tuberculosis transmission to humans.
AB - Background: A rising isolation trend of drug-resistant M. bovis from human clinical cases is documented in the literature. Here we assessed Mycobacterium tuberculosis complex isolates from cattle for drug susceptibility by the gold standard agar proportion method and a simplified resazurin microtitre assay (d-REMA). A total of 38 M. tuberculosis complex strains, including M. bovis (n = 36) and M. caprae (n = 2) isolates, from cattle in Tunisia were tested against isoniazid, rifampin, streptomycin, ethambutol, kanamycin and pyrazinamide. Results: M. caprae isolates were found to be susceptible to all test drugs. All M. bovis strains were resistant to pyrazinamide, as expected. In addition, one M. bovis isolate showed high-level resistance to streptomycin (MIC > 500.0 μg/ml). Concordant results with the two methods were found. The most common target genes associated with streptomycin resistance, namely the rrs, rpsL and gidB genes, were DNA sequenced. A non-synonymous mutation at codon 43 (K43R) was found in the rpsL gene. To the best of our knowledge, this is the first report describing the isolation of a streptomycin-resistant M. bovis isolate from animal origin. Conclusions: Antitubercular drug susceptibility testing of M. bovis isolates from animals should be performed in settings where bTB is endemic in order to estimate the magnitude of the risk of drug-resistant tuberculosis transmission to humans.
KW - Bovine tuberculosis
KW - d-REMA
KW - Mycobacterium tuberculosis complex
KW - Resazurin microtitre assay
KW - Streptomycin resistance
UR - http://www.scopus.com/inward/record.url?scp=85054251428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054251428&partnerID=8YFLogxK
U2 - 10.1186/s12917-018-1623-9
DO - 10.1186/s12917-018-1623-9
M3 - Article
C2 - 30268120
AN - SCOPUS:85054251428
VL - 14
JO - BMC Veterinary Research
JF - BMC Veterinary Research
SN - 1746-6148
IS - 1
M1 - 296
ER -