Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle 06 Biological Sciences 0604 Genetics

Saif Eddine Djemal, Cristina Camperio, Federica Armas, Mariam Siala, Salma Smaoui, Feriele Messadi-Akrout, Radhouane Gdoura, Cinzia Marianelli

Research output: Contribution to journalArticle

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Abstract

Background: A rising isolation trend of drug-resistant M. bovis from human clinical cases is documented in the literature. Here we assessed Mycobacterium tuberculosis complex isolates from cattle for drug susceptibility by the gold standard agar proportion method and a simplified resazurin microtitre assay (d-REMA). A total of 38 M. tuberculosis complex strains, including M. bovis (n = 36) and M. caprae (n = 2) isolates, from cattle in Tunisia were tested against isoniazid, rifampin, streptomycin, ethambutol, kanamycin and pyrazinamide. Results: M. caprae isolates were found to be susceptible to all test drugs. All M. bovis strains were resistant to pyrazinamide, as expected. In addition, one M. bovis isolate showed high-level resistance to streptomycin (MIC > 500.0 μg/ml). Concordant results with the two methods were found. The most common target genes associated with streptomycin resistance, namely the rrs, rpsL and gidB genes, were DNA sequenced. A non-synonymous mutation at codon 43 (K43R) was found in the rpsL gene. To the best of our knowledge, this is the first report describing the isolation of a streptomycin-resistant M. bovis isolate from animal origin. Conclusions: Antitubercular drug susceptibility testing of M. bovis isolates from animals should be performed in settings where bTB is endemic in order to estimate the magnitude of the risk of drug-resistant tuberculosis transmission to humans.

Original languageEnglish
Article number296
JournalBMC Veterinary Research
Volume14
Issue number1
DOIs
Publication statusPublished - Sep 29 2018

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Antitubercular Agents
Mycobacterium tuberculosis complex
Biological Science Disciplines
Mycobacterium bovis
Streptomycin
streptomycin
Mycobacterium tuberculosis
Biological Sciences
drugs
cattle
Pyrazinamide
Capra
testing
Goats
Pharmaceutical Preparations
Genes
isoniazid
Ethambutol
Tunisia
Multidrug-Resistant Tuberculosis

Keywords

  • Bovine tuberculosis
  • d-REMA
  • Mycobacterium tuberculosis complex
  • Resazurin microtitre assay
  • Streptomycin resistance

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Detection of a streptomycin-resistant Mycobacterium bovis strain through antitubercular drug susceptibility testing of Tunisian Mycobacterium tuberculosis complex isolates from cattle 06 Biological Sciences 0604 Genetics. / Djemal, Saif Eddine; Camperio, Cristina; Armas, Federica; Siala, Mariam; Smaoui, Salma; Messadi-Akrout, Feriele; Gdoura, Radhouane; Marianelli, Cinzia.

In: BMC Veterinary Research, Vol. 14, No. 1, 296, 29.09.2018.

Research output: Contribution to journalArticle

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