Detection of alkaline sphingomyelinase activity in human stool: Proposed role as a new diagnostic and prognostic marker of colorectal cancer

Luisa Di Marzio, Alfredo Di Leo, Benedetta Cinque, Donatella Fanini, Alessio Agnifili, Pasquale Berloco, Michele Linsalata, Dionigi Lorusso, Michele Barone, Claudio De Simone, Maria Grazia Cifone

Research output: Contribution to journalArticlepeer-review


Objectives: Intestinal alkaline sphingomyelinase, by exerting a major role in dietary sphingomyelin digestion, is responsible for the generation of messengers able to trigger the rapid turnover and apopiosis in intestinal epithelial cells. Markedly reduced mucosal alkaline sphingomyelinase activity has been associated with human colorectal neoplasms. The aim of this study was to analyze the alkaline sphingomyelinase activity in feces from healthy subjects and colorectal adenocarcinoma patients and to correlate it with the enzyme activity in intestinal tissues. Materials and Methods: The enzyme activity was measured both in the intestinal samples from 12 healthy controls and 51 patients with colorectal adenocarcinoma (tumoral and paratumoral tissue) and in the fecal samples of 34 healthy subjects and 29 patients with adenocarcinoma. The relation between sphingomyelinase activity and Dukes' stage, cell differentiation degree, age, and gender was also analyzed. Results: Alkaline sphingomyelinase was significantly decreased (P <0.001; mean reduction >90%) in tumoral intestinal mucosa of patients compared with controls independently of Dukes' stage and tumor differentiation grade. Interestingly, the enzyme activity in histologically normal paratumoral tissues was statistically lower than control samples (P <0.001). As occurs in neoplastic tissues, a relevant mean reduction (P <0.0001; almost 90%) of alkaline sphingomyelinase was revealed in stool samples from tumor patients when compared with controls. Conclusion: These findings may have implications for cancer biology and perhaps also for the design of clinical test, thus suggesting that the fecal sphingomyelinase activity could really reflect the human intestinal mucosa enzyme level and could represent a new marker for human colorectal adenocarcinoma, mainly taking into account its early appearance in intestinal neoplasms.

Original languageEnglish
Pages (from-to)856-862
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Issue number4
Publication statusPublished - Apr 2005

ASJC Scopus subject areas

  • Epidemiology
  • Oncology


Dive into the research topics of 'Detection of alkaline sphingomyelinase activity in human stool: Proposed role as a new diagnostic and prognostic marker of colorectal cancer'. Together they form a unique fingerprint.

Cite this