Detection of anti-mitochondrial antibodies by immunoprecipitation in patients with systemic sclerosis

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Abstract

Objective To describe a new immunoprecipitation pattern identified in Italian patients affected by systemic sclerosis (SSc), corresponding to the pyruvate dehydrogenase antigen complex recognized by anti-mitochondrial antibodies (AMA) in primary biliary cholangitis (PBC). Methods Autoantibodies in sera from 85 patients with SSc were tested by protein- and RNA-immunoprecipitation. Immunoprecipitation-Western blot was used to determine the identified proteins, and medical records re-evaluated for liver function tests and PBC. Results In 13/85 (15%) SSc sera, a unique set of 75-50-40-34 kD proteins that had not been previously reported, was noted. The four proteins were identified as the proteins X/E3BP, E1α E1β and E2/E3 of the pyruvate dehydrogenase antigen complex by immunoprecipitation-Western blot. From clinical record evaluation, 9/13 (69%) SSc patients with this new pattern were positive for AMA by routine indirect immunofluorescence, and 7/13 (54%) had a diagnosis of PBC, while 4/13 (31%) manifested no biochemical signs of cholestasis. Twelve of 13 patients with SSc and AMA by immunoprecipitation have a limited cutaneous form of SSc and anti-centromere antibodies. Conclusions We describe a pattern of 4 proteins in 15% of SSc patients, identified for the first time by protein-immunoprecipitation. This pattern corresponds to serum AMA against the pyruvate dehydrogenase antigen complex and it must be considered in the interpretation of protein-immunoprecipitation results.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJournal of Immunological Methods
Volume452
DOIs
Publication statusPublished - Jan 1 2018

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Systemic Scleroderma
Immunoprecipitation
Anti-Idiotypic Antibodies
Pyruvate Dehydrogenase Complex
Cholangitis
Proteins
Antigens
Western Blotting
Serum
Centromere
Liver Function Tests
Cholestasis
Indirect Fluorescent Antibody Technique
Autoantibodies
Medical Records
RNA
Skin

Keywords

  • Autoimmune cholangitis
  • Autoimmunity
  • Immunoprecipitation
  • Pyruvate dehydrogenase
  • Scleroderma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

@article{0332dd689a834261b09ad0ab7ae53c69,
title = "Detection of anti-mitochondrial antibodies by immunoprecipitation in patients with systemic sclerosis",
abstract = "Objective To describe a new immunoprecipitation pattern identified in Italian patients affected by systemic sclerosis (SSc), corresponding to the pyruvate dehydrogenase antigen complex recognized by anti-mitochondrial antibodies (AMA) in primary biliary cholangitis (PBC). Methods Autoantibodies in sera from 85 patients with SSc were tested by protein- and RNA-immunoprecipitation. Immunoprecipitation-Western blot was used to determine the identified proteins, and medical records re-evaluated for liver function tests and PBC. Results In 13/85 (15{\%}) SSc sera, a unique set of 75-50-40-34 kD proteins that had not been previously reported, was noted. The four proteins were identified as the proteins X/E3BP, E1α E1β and E2/E3 of the pyruvate dehydrogenase antigen complex by immunoprecipitation-Western blot. From clinical record evaluation, 9/13 (69{\%}) SSc patients with this new pattern were positive for AMA by routine indirect immunofluorescence, and 7/13 (54{\%}) had a diagnosis of PBC, while 4/13 (31{\%}) manifested no biochemical signs of cholestasis. Twelve of 13 patients with SSc and AMA by immunoprecipitation have a limited cutaneous form of SSc and anti-centromere antibodies. Conclusions We describe a pattern of 4 proteins in 15{\%} of SSc patients, identified for the first time by protein-immunoprecipitation. This pattern corresponds to serum AMA against the pyruvate dehydrogenase antigen complex and it must be considered in the interpretation of protein-immunoprecipitation results.",
keywords = "Autoimmune cholangitis, Autoimmunity, Immunoprecipitation, Pyruvate dehydrogenase, Scleroderma",
author = "Angela Ceribelli and Natasa Isailovic and {De Santis}, Maria and Elena Generali and Minoru Satoh and Carlo Selmi",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.jim.2017.10.001",
language = "English",
volume = "452",
pages = "1--5",
journal = "Journal of Immunological Methods",
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TY - JOUR

T1 - Detection of anti-mitochondrial antibodies by immunoprecipitation in patients with systemic sclerosis

AU - Ceribelli, Angela

AU - Isailovic, Natasa

AU - De Santis, Maria

AU - Generali, Elena

AU - Satoh, Minoru

AU - Selmi, Carlo

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objective To describe a new immunoprecipitation pattern identified in Italian patients affected by systemic sclerosis (SSc), corresponding to the pyruvate dehydrogenase antigen complex recognized by anti-mitochondrial antibodies (AMA) in primary biliary cholangitis (PBC). Methods Autoantibodies in sera from 85 patients with SSc were tested by protein- and RNA-immunoprecipitation. Immunoprecipitation-Western blot was used to determine the identified proteins, and medical records re-evaluated for liver function tests and PBC. Results In 13/85 (15%) SSc sera, a unique set of 75-50-40-34 kD proteins that had not been previously reported, was noted. The four proteins were identified as the proteins X/E3BP, E1α E1β and E2/E3 of the pyruvate dehydrogenase antigen complex by immunoprecipitation-Western blot. From clinical record evaluation, 9/13 (69%) SSc patients with this new pattern were positive for AMA by routine indirect immunofluorescence, and 7/13 (54%) had a diagnosis of PBC, while 4/13 (31%) manifested no biochemical signs of cholestasis. Twelve of 13 patients with SSc and AMA by immunoprecipitation have a limited cutaneous form of SSc and anti-centromere antibodies. Conclusions We describe a pattern of 4 proteins in 15% of SSc patients, identified for the first time by protein-immunoprecipitation. This pattern corresponds to serum AMA against the pyruvate dehydrogenase antigen complex and it must be considered in the interpretation of protein-immunoprecipitation results.

AB - Objective To describe a new immunoprecipitation pattern identified in Italian patients affected by systemic sclerosis (SSc), corresponding to the pyruvate dehydrogenase antigen complex recognized by anti-mitochondrial antibodies (AMA) in primary biliary cholangitis (PBC). Methods Autoantibodies in sera from 85 patients with SSc were tested by protein- and RNA-immunoprecipitation. Immunoprecipitation-Western blot was used to determine the identified proteins, and medical records re-evaluated for liver function tests and PBC. Results In 13/85 (15%) SSc sera, a unique set of 75-50-40-34 kD proteins that had not been previously reported, was noted. The four proteins were identified as the proteins X/E3BP, E1α E1β and E2/E3 of the pyruvate dehydrogenase antigen complex by immunoprecipitation-Western blot. From clinical record evaluation, 9/13 (69%) SSc patients with this new pattern were positive for AMA by routine indirect immunofluorescence, and 7/13 (54%) had a diagnosis of PBC, while 4/13 (31%) manifested no biochemical signs of cholestasis. Twelve of 13 patients with SSc and AMA by immunoprecipitation have a limited cutaneous form of SSc and anti-centromere antibodies. Conclusions We describe a pattern of 4 proteins in 15% of SSc patients, identified for the first time by protein-immunoprecipitation. This pattern corresponds to serum AMA against the pyruvate dehydrogenase antigen complex and it must be considered in the interpretation of protein-immunoprecipitation results.

KW - Autoimmune cholangitis

KW - Autoimmunity

KW - Immunoprecipitation

KW - Pyruvate dehydrogenase

KW - Scleroderma

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