Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis

Amelie Simon, Jean Francois Subra, Philippe Guilpain, Pascale Jeannin, Pascale Pignon, Simon Blanchard, Erwan Garo, Sebastien Jaillon, Alain Chevailler, Gilles Renier, Xavier Puechal, Barbara Bottazzi, Alberto Mantovani, Yves Delneste, Jean Francois Augusto

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives Pentraxin 3 (PTX3), in common with myeloperoxidase and proteinase 3, is stored in human neutrophil granules and is expressed on apoptotic neutrophil surface. We therefore investigated the presence of anti-PTX3 autoantibodies (aAbs) in the sera of antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) patients. Methods Presence of anti-PTX3 autoantibodies was analysed by a specific enzyme-linked immunosorbent assay in sera from 150 patients with microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA), and eosinophilic granulomatosis with polyangiitis (EGPA), and in sera of 227 healthy subjects (HS), 40 systemic sclerosis (SSc) patients, and 25 giant cell arteritis patients (GCA). Using indirect immunofluorescence on fixed human neutrophils, we also analyzed the staining pattern associated with the presence of anti-PTX3 aAbs. Results Anti-PTX3 aAbs were detected in 56 of 150 (37.3%) of the AAV patients (versus 12 of 227 (5.3%) of HS, p

Original languageEnglish
Article numbere0147091
JournalPLoS One
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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