Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis

Amelie Simon, Jean Francois Subra, Philippe Guilpain, Pascale Jeannin, Pascale Pignon, Simon Blanchard, Erwan Garo, Sebastien Jaillon, Alain Chevailler, Gilles Renier, Xavier Puechal, Barbara Bottazzi, Alberto Mantovani, Yves Delneste, Jean Francois Augusto

Research output: Contribution to journalArticle

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Abstract

Objectives Pentraxin 3 (PTX3), in common with myeloperoxidase and proteinase 3, is stored in human neutrophil granules and is expressed on apoptotic neutrophil surface. We therefore investigated the presence of anti-PTX3 autoantibodies (aAbs) in the sera of antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) patients. Methods Presence of anti-PTX3 autoantibodies was analysed by a specific enzyme-linked immunosorbent assay in sera from 150 patients with microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA), and eosinophilic granulomatosis with polyangiitis (EGPA), and in sera of 227 healthy subjects (HS), 40 systemic sclerosis (SSc) patients, and 25 giant cell arteritis patients (GCA). Using indirect immunofluorescence on fixed human neutrophils, we also analyzed the staining pattern associated with the presence of anti-PTX3 aAbs. Results Anti-PTX3 aAbs were detected in 56 of 150 (37.3%) of the AAV patients (versus 12 of 227 (5.3%) of HS, p

Original languageEnglish
Article numbere0147091
JournalPLoS One
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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vasculitis
Antineutrophil Cytoplasmic Antibodies
autoantibodies
Vasculitis
Autoantibodies
antibodies
neutrophils
Granulomatosis with Polyangiitis
Neutrophils
Healthy Volunteers
Serum
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis
Microscopic Polyangiitis
Myeloblastin
arteritis
Immunosorbents
Giant Cell Arteritis
myeloperoxidase
Systemic Scleroderma
sclerosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Simon, A., Subra, J. F., Guilpain, P., Jeannin, P., Pignon, P., Blanchard, S., ... Augusto, J. F. (2016). Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis. PLoS One, 11(1), [e0147091]. https://doi.org/10.1371/journal.pone.0147091

Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis. / Simon, Amelie; Subra, Jean Francois; Guilpain, Philippe; Jeannin, Pascale; Pignon, Pascale; Blanchard, Simon; Garo, Erwan; Jaillon, Sebastien; Chevailler, Alain; Renier, Gilles; Puechal, Xavier; Bottazzi, Barbara; Mantovani, Alberto; Delneste, Yves; Augusto, Jean Francois.

In: PLoS One, Vol. 11, No. 1, e0147091, 01.01.2016.

Research output: Contribution to journalArticle

Simon, A, Subra, JF, Guilpain, P, Jeannin, P, Pignon, P, Blanchard, S, Garo, E, Jaillon, S, Chevailler, A, Renier, G, Puechal, X, Bottazzi, B, Mantovani, A, Delneste, Y & Augusto, JF 2016, 'Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis', PLoS One, vol. 11, no. 1, e0147091. https://doi.org/10.1371/journal.pone.0147091
Simon A, Subra JF, Guilpain P, Jeannin P, Pignon P, Blanchard S et al. Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis. PLoS One. 2016 Jan 1;11(1). e0147091. https://doi.org/10.1371/journal.pone.0147091
Simon, Amelie ; Subra, Jean Francois ; Guilpain, Philippe ; Jeannin, Pascale ; Pignon, Pascale ; Blanchard, Simon ; Garo, Erwan ; Jaillon, Sebastien ; Chevailler, Alain ; Renier, Gilles ; Puechal, Xavier ; Bottazzi, Barbara ; Mantovani, Alberto ; Delneste, Yves ; Augusto, Jean Francois. / Detection of anti-pentraxin-3 autoantibodies in ANCA-associated vasculitis. In: PLoS One. 2016 ; Vol. 11, No. 1.
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