Detection of anti-pituitary autoantibodies by immunoblotting

Patricia Crock, Mario Salvi, Ann Miller, Jack Wall, Harvey Guyda

Research output: Contribution to journalArticle

Abstract

A new approach to the detection of anti-pituitary autoantibodies by immunoblotting is presented. This method distinguishes pituitary membrane fraction from cytosolic fraction autoantigens and characterizes them by their molecular weight. A 45 kDa pituitary specific membrane protein was identified as an autoantigen in one of 19 patients with idiopathic growth hormone deficiency and the empty sella syndrome. A 43 kDa membrane protein in pituitary and brain was identified as an autoantigen in one other patient with idiopathic growth hormone deficiency and in one of 14 patients with secondary growth hormone deficiency. These autoantibodies were not seen in any of 27 control subjects. Anti-pituitary autoantibodies can be demonstrated by immunoblotting at titres of up to 1 1000. We conclude that immunoblotting is a useful method for the detection of anti-pituitary autoantibodies.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalJournal of Immunological Methods
Volume162
Issue number1
DOIs
Publication statusPublished - Jun 4 1993

Keywords

  • Anti-pituitary autoantibody
  • Empty sella syndrome
  • Growth hormone deficiency
  • Immunoblotting

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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