Abstract
The exposure to DNA reactive carcinogens is known to elicit a specific humoral immunological response, with the production of antibodies toward the carcinogen adducts. Consequently, the presence of circulating anti-carcinogen antibodies has been proposed as a marker of carcinogen exposure, and as a potential modulating factor in chemical carcinogenesis. In this work, the presence of serum antibodies to 7β,8α-dihydroxy-9α10α-epoxy-7,8,9,10- tetrahydrobenzo[a]pyrene-DNA (BPDE-DNA) adducts was determined in two groups of workers occupationally exposed to low doses of polycyclic aromatic hydrocarbons (PAHs), i.e. policemen (194 subjects) and workers in the aluminum industry (105 subjects). Specific anti BPDE-DNA antibodies were detected in 5.7% (11/194) of policemen and 13.3% (14/105) of aluminium industry workers. Among policemen, a small, not significant (p=0.09), prevalence of positives was observed in traffic wardens compared to office workers. A borderline significant (p=0.052) prevalence of positives was also observed in heavy smokers compared to light smokers among aluminium industry workers. These results basically support previous findings on the association between chronic exposure to polycyclic aromatic hydrocarbons and formation of anti- BPDE-DNA antibodies, even though such association appears to be weak, possibly biased by individual factors which are still largely unidentified.
| Original language | English |
|---|---|
| Pages (from-to) | 359-364 |
| Number of pages | 6 |
| Journal | Journal of Experimental and Clinical Cancer Research |
| Volume | 20 |
| Issue number | 3 |
| Publication status | Published - 2001 |
Fingerprint
Keywords
- Benzo(a)pyrene
- Exposure biomarker
- Specific antibodies
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Detection of antibodies to the Benzo(a)pyrene diol epoxide-DNA adducts in sera from individuals exposed to low doses of polycyclic aromatic hydrocarbons. / Galati, R.; Zijno, A.; Crebelli, R.; Falasca, G.; Tomei, F.; Iecher, F.; Carta, P.; Verdina, A.
In: Journal of Experimental and Clinical Cancer Research, Vol. 20, No. 3, 2001, p. 359-364.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of antibodies to the Benzo(a)pyrene diol epoxide-DNA adducts in sera from individuals exposed to low doses of polycyclic aromatic hydrocarbons
AU - Galati, R.
AU - Zijno, A.
AU - Crebelli, R.
AU - Falasca, G.
AU - Tomei, F.
AU - Iecher, F.
AU - Carta, P.
AU - Verdina, A.
PY - 2001
Y1 - 2001
N2 - The exposure to DNA reactive carcinogens is known to elicit a specific humoral immunological response, with the production of antibodies toward the carcinogen adducts. Consequently, the presence of circulating anti-carcinogen antibodies has been proposed as a marker of carcinogen exposure, and as a potential modulating factor in chemical carcinogenesis. In this work, the presence of serum antibodies to 7β,8α-dihydroxy-9α10α-epoxy-7,8,9,10- tetrahydrobenzo[a]pyrene-DNA (BPDE-DNA) adducts was determined in two groups of workers occupationally exposed to low doses of polycyclic aromatic hydrocarbons (PAHs), i.e. policemen (194 subjects) and workers in the aluminum industry (105 subjects). Specific anti BPDE-DNA antibodies were detected in 5.7% (11/194) of policemen and 13.3% (14/105) of aluminium industry workers. Among policemen, a small, not significant (p=0.09), prevalence of positives was observed in traffic wardens compared to office workers. A borderline significant (p=0.052) prevalence of positives was also observed in heavy smokers compared to light smokers among aluminium industry workers. These results basically support previous findings on the association between chronic exposure to polycyclic aromatic hydrocarbons and formation of anti- BPDE-DNA antibodies, even though such association appears to be weak, possibly biased by individual factors which are still largely unidentified.
AB - The exposure to DNA reactive carcinogens is known to elicit a specific humoral immunological response, with the production of antibodies toward the carcinogen adducts. Consequently, the presence of circulating anti-carcinogen antibodies has been proposed as a marker of carcinogen exposure, and as a potential modulating factor in chemical carcinogenesis. In this work, the presence of serum antibodies to 7β,8α-dihydroxy-9α10α-epoxy-7,8,9,10- tetrahydrobenzo[a]pyrene-DNA (BPDE-DNA) adducts was determined in two groups of workers occupationally exposed to low doses of polycyclic aromatic hydrocarbons (PAHs), i.e. policemen (194 subjects) and workers in the aluminum industry (105 subjects). Specific anti BPDE-DNA antibodies were detected in 5.7% (11/194) of policemen and 13.3% (14/105) of aluminium industry workers. Among policemen, a small, not significant (p=0.09), prevalence of positives was observed in traffic wardens compared to office workers. A borderline significant (p=0.052) prevalence of positives was also observed in heavy smokers compared to light smokers among aluminium industry workers. These results basically support previous findings on the association between chronic exposure to polycyclic aromatic hydrocarbons and formation of anti- BPDE-DNA antibodies, even though such association appears to be weak, possibly biased by individual factors which are still largely unidentified.
KW - Benzo(a)pyrene
KW - Exposure biomarker
KW - Specific antibodies
UR - http://www.scopus.com/inward/record.url?scp=0034778967&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034778967&partnerID=8YFLogxK
M3 - Article
C2 - 11718215
AN - SCOPUS:0034778967
VL - 20
SP - 359
EP - 364
JO - Journal of Experimental and Clinical Cancer Research
JF - Journal of Experimental and Clinical Cancer Research
SN - 0392-9078
IS - 3
ER -