Detection of basophils growing in semisolid agar culture

M. Aglietta, G. Camussi, W. Piacibello

Research output: Contribution to journalArticlepeer-review

Abstract

The production of basophils in semisolid agar cultures from normal and chronic myeloid leukemia (CML) committed granulocyte-macrophage precursors was investigated using an original whole-dish staining technique with toluidine blue which produces a specific metachromasia in basophils. As additional proof of the basophilic nature of metachromatic cells, their degranulation after challenge with C5a anaphylotoxin and Synachten was observed. Our studies show that few basophils are produced in cultures from normal bone marrow. CML CFU(c) produce more basophils, their number being roughly correlated with the degree of basophilia. We observed only clusters composed by a pure basophilic population, while larger aggregates in which basophils could be detected were composed also by other granulocytic cells.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalExperimental Hematology
Volume9
Issue number2
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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