Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus

Research output: Contribution to journalArticle

Abstract

The formation of biofilm represents an important virulence factor of certain strains of Staphylococcus epidermidis and S. aureus. The ability of bacteria to aggregate, forming biofilms, is strictly related to the capacity of producing an extracellular mucoid substance often referred to as slime, whose main component is of polysaccharidic nature and consists of glycosaminoglycans. In recent years, new molecular techniques based on PCR have come alongside more traditional methods for identification of virulent biofilm-forming strains. The detection of the genes governing the production of such extracellular polysaccharide and, in particular, the icaA, the icaC and the icaD genes, provides us with a rapid and accurate technique for strain characterization. However, well-established methods, such as the Congo red agar test are still needed in order to confirm the phenotypic expression in the case of possible phase-variant strains. In future, the complete knowledge of the genetic mechanisms of phenotype modulation, comprehending all regulatory genes, could permit the characterization of the isolates just by molecular means in a single step.

Original languageEnglish
Pages (from-to)478-484
Number of pages7
JournalExpert Review of Molecular Diagnostics
Volume2
Issue number5
Publication statusPublished - Sep 2002

Fingerprint

Staphylococcus epidermidis
Biofilms
Congo Red
Virulence Factors
Regulator Genes
Glycosaminoglycans
Genes
Agar
Polysaccharides
Bacteria
Phenotype
Polymerase Chain Reaction

Keywords

  • Biofilm
  • Congo red agar test
  • Detection techniques
  • ica genes
  • Microtiter plate test
  • Slime Staphylococcus aureus
  • Staphylocuccus epidermidis
  • Virulence factors

ASJC Scopus subject areas

  • Genetics

Cite this

Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus. / Arciola, Carla Renata; Campoccia, Davide; Montanaro, Lucio.

In: Expert Review of Molecular Diagnostics, Vol. 2, No. 5, 09.2002, p. 478-484.

Research output: Contribution to journalArticle

@article{bea64f086d2d4dc4b951db65c1367e05,
title = "Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus",
abstract = "The formation of biofilm represents an important virulence factor of certain strains of Staphylococcus epidermidis and S. aureus. The ability of bacteria to aggregate, forming biofilms, is strictly related to the capacity of producing an extracellular mucoid substance often referred to as slime, whose main component is of polysaccharidic nature and consists of glycosaminoglycans. In recent years, new molecular techniques based on PCR have come alongside more traditional methods for identification of virulent biofilm-forming strains. The detection of the genes governing the production of such extracellular polysaccharide and, in particular, the icaA, the icaC and the icaD genes, provides us with a rapid and accurate technique for strain characterization. However, well-established methods, such as the Congo red agar test are still needed in order to confirm the phenotypic expression in the case of possible phase-variant strains. In future, the complete knowledge of the genetic mechanisms of phenotype modulation, comprehending all regulatory genes, could permit the characterization of the isolates just by molecular means in a single step.",
keywords = "Biofilm, Congo red agar test, Detection techniques, ica genes, Microtiter plate test, Slime Staphylococcus aureus, Staphylocuccus epidermidis, Virulence factors",
author = "Arciola, {Carla Renata} and Davide Campoccia and Lucio Montanaro",
year = "2002",
month = "9",
language = "English",
volume = "2",
pages = "478--484",
journal = "Expert Review of Molecular Diagnostics",
issn = "1473-7159",
publisher = "Expert Reviews Ltd.",
number = "5",

}

TY - JOUR

T1 - Detection of biofilm-forming strains of Staphylococcus epidermidis and S. aureus

AU - Arciola, Carla Renata

AU - Campoccia, Davide

AU - Montanaro, Lucio

PY - 2002/9

Y1 - 2002/9

N2 - The formation of biofilm represents an important virulence factor of certain strains of Staphylococcus epidermidis and S. aureus. The ability of bacteria to aggregate, forming biofilms, is strictly related to the capacity of producing an extracellular mucoid substance often referred to as slime, whose main component is of polysaccharidic nature and consists of glycosaminoglycans. In recent years, new molecular techniques based on PCR have come alongside more traditional methods for identification of virulent biofilm-forming strains. The detection of the genes governing the production of such extracellular polysaccharide and, in particular, the icaA, the icaC and the icaD genes, provides us with a rapid and accurate technique for strain characterization. However, well-established methods, such as the Congo red agar test are still needed in order to confirm the phenotypic expression in the case of possible phase-variant strains. In future, the complete knowledge of the genetic mechanisms of phenotype modulation, comprehending all regulatory genes, could permit the characterization of the isolates just by molecular means in a single step.

AB - The formation of biofilm represents an important virulence factor of certain strains of Staphylococcus epidermidis and S. aureus. The ability of bacteria to aggregate, forming biofilms, is strictly related to the capacity of producing an extracellular mucoid substance often referred to as slime, whose main component is of polysaccharidic nature and consists of glycosaminoglycans. In recent years, new molecular techniques based on PCR have come alongside more traditional methods for identification of virulent biofilm-forming strains. The detection of the genes governing the production of such extracellular polysaccharide and, in particular, the icaA, the icaC and the icaD genes, provides us with a rapid and accurate technique for strain characterization. However, well-established methods, such as the Congo red agar test are still needed in order to confirm the phenotypic expression in the case of possible phase-variant strains. In future, the complete knowledge of the genetic mechanisms of phenotype modulation, comprehending all regulatory genes, could permit the characterization of the isolates just by molecular means in a single step.

KW - Biofilm

KW - Congo red agar test

KW - Detection techniques

KW - ica genes

KW - Microtiter plate test

KW - Slime Staphylococcus aureus

KW - Staphylocuccus epidermidis

KW - Virulence factors

UR - http://www.scopus.com/inward/record.url?scp=0036714986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036714986&partnerID=8YFLogxK

M3 - Article

C2 - 12271819

AN - SCOPUS:0036714986

VL - 2

SP - 478

EP - 484

JO - Expert Review of Molecular Diagnostics

JF - Expert Review of Molecular Diagnostics

SN - 1473-7159

IS - 5

ER -