Detection of biomolecules using light scattering

Carlo Morasso, Furio Gramatica, Tommaso Bellini, Davide Prosperi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, a straightforward method based on elastic light scattering is shown to provide a sensitive and reliable tool for the quantitative determination of receptor-ligand interactions that occur at the surface of suitably designed core-shell nanoparticles. The assay makes use of monodisperse nanocolloids that have minimal optical contrast with the aqueous environment. By properly coating the particles with an appropriate receptor and unreactive amphiphiles, we developed a hybrid system that combines the availability of standard ligands with the necessary bioinvisibility towards the accidental adsorption of nonspecific macromolecules. This system has been used for the study of the interactions between small molecules (Vancomycin-Lipid 2), and between proteins (Avidin-biotinnylated IgG.

Original languageEnglish
Title of host publication2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
DOIs
Publication statusPublished - 2011
Event2011 International Workshop on Biophotonics, BIOPHOTONICS 2011 - Parma, Italy
Duration: Jun 8 2011Jun 10 2011

Other

Other2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
CountryItaly
CityParma
Period6/8/116/10/11

Fingerprint

Biomolecules
Light scattering
Ligands
Light
Amphiphiles
Elastic scattering
Avidin
Vancomycin
Hybrid systems
Macromolecules
Nanoparticles
Lipids
Adsorption
Assays
Immunoglobulin G
Availability
Proteins
Coatings
Molecules

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Biomedical Engineering

Cite this

Morasso, C., Gramatica, F., Bellini, T., & Prosperi, D. (2011). Detection of biomolecules using light scattering. In 2011 International Workshop on Biophotonics, BIOPHOTONICS 2011 [5954839] https://doi.org/10.1109/IWBP.2011.5954839

Detection of biomolecules using light scattering. / Morasso, Carlo; Gramatica, Furio; Bellini, Tommaso; Prosperi, Davide.

2011 International Workshop on Biophotonics, BIOPHOTONICS 2011. 2011. 5954839.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Morasso, C, Gramatica, F, Bellini, T & Prosperi, D 2011, Detection of biomolecules using light scattering. in 2011 International Workshop on Biophotonics, BIOPHOTONICS 2011., 5954839, 2011 International Workshop on Biophotonics, BIOPHOTONICS 2011, Parma, Italy, 6/8/11. https://doi.org/10.1109/IWBP.2011.5954839
Morasso C, Gramatica F, Bellini T, Prosperi D. Detection of biomolecules using light scattering. In 2011 International Workshop on Biophotonics, BIOPHOTONICS 2011. 2011. 5954839 https://doi.org/10.1109/IWBP.2011.5954839
Morasso, Carlo ; Gramatica, Furio ; Bellini, Tommaso ; Prosperi, Davide. / Detection of biomolecules using light scattering. 2011 International Workshop on Biophotonics, BIOPHOTONICS 2011. 2011.
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