Abstract
Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 in whole blood and urinary bladder tumours of adult cattle in an attempt to better understand the biological role of circulating BPV-2. Peripheral blood samples were collected from 78 cattle clinically suffering from a severe chronic enzootic haematuria. Circulating BPV-2 DNA was detected in 61 of them and in two blood samples from healthy cows. Fifty of the affected animals were slaughtered at public slaughterhouses and neoplastic proliferations in the urinary bladder were detected in all of them. BPV-2 DNA was amplified and sequenced in 78% of urinary bladder tumour samples and in 38.9% of normal samples as a control. Circulating episomal BPV-2 DNA was detected in 78.2% of the blood samples. Simultaneous presence of BPV-2 DNA in neoplastic bladder and blood samples was detected in 37 animals. Specific viral E5 mRNA and E5 oncoprotein were also detected in blood by RT-PCR and Western blot/ immunocytochemistry, respectively. It is likely that BPV-2 can persist and be maintained in an active status in the bloodstream, in particular in the lymphocytes, as a reservoir of viral infection that, in the presence of co-carcinogens, may cause the development of urinary bladder tumours.
| Original language | English |
|---|---|
| Pages (from-to) | 3027-3033 |
| Number of pages | 7 |
| Journal | Journal of General Virology |
| Volume | 89 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2008 |
Fingerprint
ASJC Scopus subject areas
- Virology
Cite this
Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours : Possible biological role. / Roperto, Sante; Brun, Roberto; Paolini, Francesca; Urraro, Chiara; Russo, Valeria; Borzacchiello, Giuseppe; Pagnini, Ugo; Raso, Cinzia; Rizzo, Consuelo; Roperto, Franco; Venuti, Aldo.
In: Journal of General Virology, Vol. 89, No. 12, 2008, p. 3027-3033.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours
T2 - Possible biological role
AU - Roperto, Sante
AU - Brun, Roberto
AU - Paolini, Francesca
AU - Urraro, Chiara
AU - Russo, Valeria
AU - Borzacchiello, Giuseppe
AU - Pagnini, Ugo
AU - Raso, Cinzia
AU - Rizzo, Consuelo
AU - Roperto, Franco
AU - Venuti, Aldo
PY - 2008
Y1 - 2008
N2 - Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 in whole blood and urinary bladder tumours of adult cattle in an attempt to better understand the biological role of circulating BPV-2. Peripheral blood samples were collected from 78 cattle clinically suffering from a severe chronic enzootic haematuria. Circulating BPV-2 DNA was detected in 61 of them and in two blood samples from healthy cows. Fifty of the affected animals were slaughtered at public slaughterhouses and neoplastic proliferations in the urinary bladder were detected in all of them. BPV-2 DNA was amplified and sequenced in 78% of urinary bladder tumour samples and in 38.9% of normal samples as a control. Circulating episomal BPV-2 DNA was detected in 78.2% of the blood samples. Simultaneous presence of BPV-2 DNA in neoplastic bladder and blood samples was detected in 37 animals. Specific viral E5 mRNA and E5 oncoprotein were also detected in blood by RT-PCR and Western blot/ immunocytochemistry, respectively. It is likely that BPV-2 can persist and be maintained in an active status in the bloodstream, in particular in the lymphocytes, as a reservoir of viral infection that, in the presence of co-carcinogens, may cause the development of urinary bladder tumours.
AB - Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 in whole blood and urinary bladder tumours of adult cattle in an attempt to better understand the biological role of circulating BPV-2. Peripheral blood samples were collected from 78 cattle clinically suffering from a severe chronic enzootic haematuria. Circulating BPV-2 DNA was detected in 61 of them and in two blood samples from healthy cows. Fifty of the affected animals were slaughtered at public slaughterhouses and neoplastic proliferations in the urinary bladder were detected in all of them. BPV-2 DNA was amplified and sequenced in 78% of urinary bladder tumour samples and in 38.9% of normal samples as a control. Circulating episomal BPV-2 DNA was detected in 78.2% of the blood samples. Simultaneous presence of BPV-2 DNA in neoplastic bladder and blood samples was detected in 37 animals. Specific viral E5 mRNA and E5 oncoprotein were also detected in blood by RT-PCR and Western blot/ immunocytochemistry, respectively. It is likely that BPV-2 can persist and be maintained in an active status in the bloodstream, in particular in the lymphocytes, as a reservoir of viral infection that, in the presence of co-carcinogens, may cause the development of urinary bladder tumours.
UR - http://www.scopus.com/inward/record.url?scp=58149376711&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149376711&partnerID=8YFLogxK
U2 - 10.1099/vir.0.2008/004457-0
DO - 10.1099/vir.0.2008/004457-0
M3 - Article
C2 - 19008389
AN - SCOPUS:58149376711
VL - 89
SP - 3027
EP - 3033
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 12
ER -