Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours: Possible biological role

Sante Roperto, Roberto Brun, Francesca Paolini, Chiara Urraro, Valeria Russo, Giuseppe Borzacchiello, Ugo Pagnini, Cinzia Raso, Consuelo Rizzo, Franco Roperto, Aldo Venuti

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60 Citations (Scopus)

Abstract

Bovine papillomavirus type 2 (BPV-2) infection has been associated with urinary bladder tumours in adult cattle grazing on bracken fern-infested land. In this study, we investigated the simultaneous presence of BPV-2 in whole blood and urinary bladder tumours of adult cattle in an attempt to better understand the biological role of circulating BPV-2. Peripheral blood samples were collected from 78 cattle clinically suffering from a severe chronic enzootic haematuria. Circulating BPV-2 DNA was detected in 61 of them and in two blood samples from healthy cows. Fifty of the affected animals were slaughtered at public slaughterhouses and neoplastic proliferations in the urinary bladder were detected in all of them. BPV-2 DNA was amplified and sequenced in 78% of urinary bladder tumour samples and in 38.9% of normal samples as a control. Circulating episomal BPV-2 DNA was detected in 78.2% of the blood samples. Simultaneous presence of BPV-2 DNA in neoplastic bladder and blood samples was detected in 37 animals. Specific viral E5 mRNA and E5 oncoprotein were also detected in blood by RT-PCR and Western blot/ immunocytochemistry, respectively. It is likely that BPV-2 can persist and be maintained in an active status in the bloodstream, in particular in the lymphocytes, as a reservoir of viral infection that, in the presence of co-carcinogens, may cause the development of urinary bladder tumours.

Original languageEnglish
Pages (from-to)3027-3033
Number of pages7
JournalJournal of General Virology
Volume89
Issue number12
DOIs
Publication statusPublished - 2008

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Bovine papillomavirus 1
Urinary Bladder Neoplasms
Urinary Bladder
DNA
Pteridium
Abattoirs
Papillomavirus Infections
Oncogene Proteins
Hematuria
Virus Diseases
Carcinogens
Western Blotting
Immunohistochemistry
Lymphocytes
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Virology

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Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours : Possible biological role. / Roperto, Sante; Brun, Roberto; Paolini, Francesca; Urraro, Chiara; Russo, Valeria; Borzacchiello, Giuseppe; Pagnini, Ugo; Raso, Cinzia; Rizzo, Consuelo; Roperto, Franco; Venuti, Aldo.

In: Journal of General Virology, Vol. 89, No. 12, 2008, p. 3027-3033.

Research output: Contribution to journalArticle

Roperto, S, Brun, R, Paolini, F, Urraro, C, Russo, V, Borzacchiello, G, Pagnini, U, Raso, C, Rizzo, C, Roperto, F & Venuti, A 2008, 'Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours: Possible biological role', Journal of General Virology, vol. 89, no. 12, pp. 3027-3033. https://doi.org/10.1099/vir.0.2008/004457-0
Roperto, Sante ; Brun, Roberto ; Paolini, Francesca ; Urraro, Chiara ; Russo, Valeria ; Borzacchiello, Giuseppe ; Pagnini, Ugo ; Raso, Cinzia ; Rizzo, Consuelo ; Roperto, Franco ; Venuti, Aldo. / Detection of bovine papillomavirus type 2 in the peripheral blood of cattle with urinary bladder tumours : Possible biological role. In: Journal of General Virology. 2008 ; Vol. 89, No. 12. pp. 3027-3033.
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