Detection of Chlamydiae pneumoniae but not Helicobacter pylori DNA in atherosclerosis plaques

Maria Pina Dore, Antonia R. Sepulveda, Piero P. Bacciu, Francesco Blasi, Luigi Simula, Lea Marras, Davide Piccolo, Giovanni B. Cherchi, David Y. Graham, Giuseppe Realdi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Chronic infections have been associated with cardiovascular disease. We used bacterial culture, polymerase chain reaction (PCR), and immunohistochemical staining with anti-vacA and anticagA antibodies to search for Helicobacter pylori and Chlamydiae pneumoniae in atherosclerotic plaques obtained at endarterectomy. Serum IgG antibodies to H. pylori and C. pneumoniae were also determined. Thirty-two patients were enrolled. Anti-H. pylori and anti-C. pneumoniae IgG were present in 72% and 81%, respectively. Culture and PCR for H. pylori of vessel walls and plaques were negative. Atherosclerotic plaque and normal vessel sections from H. pylori-negative and- positive patients showed reactivity with anti-vacA and anti-cagA antibodies. C. pneumoniae DNA was amplified in three atherosclerotic lesions. These findings suggest that the association between H. pylori infection and atherosclerosis does not result from continuing direct effects of H. pylori antigens in the vessel walls. Antigens within vessel atherosclerotic plaques cross-react with H. pylori virulence factors and could act as cofactors in determining instability for the atherosclerotic plaques.

Original languageEnglish
Pages (from-to)945-951
Number of pages7
JournalDigestive Diseases and Sciences
Volume48
Issue number5
DOIs
Publication statusPublished - May 1 2003

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Chlamydophila pneumoniae
Helicobacter pylori
Atherosclerosis
Atherosclerotic Plaques
DNA
Immunoglobulin G
Polymerase Chain Reaction
Endarterectomy
Antibodies
Helicobacter Infections
Virulence Factors
Anti-Idiotypic Antibodies
Cardiovascular Diseases
Staining and Labeling
Antigens
Infection
Serum

Keywords

  • Atherosclerosis
  • Chlamydiae pneumoniae
  • Dyspepsia
  • Helicobacter pylori infection
  • Mimicry

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Detection of Chlamydiae pneumoniae but not Helicobacter pylori DNA in atherosclerosis plaques. / Dore, Maria Pina; Sepulveda, Antonia R.; Bacciu, Piero P.; Blasi, Francesco; Simula, Luigi; Marras, Lea; Piccolo, Davide; Cherchi, Giovanni B.; Graham, David Y.; Realdi, Giuseppe.

In: Digestive Diseases and Sciences, Vol. 48, No. 5, 01.05.2003, p. 945-951.

Research output: Contribution to journalArticle

Dore, MP, Sepulveda, AR, Bacciu, PP, Blasi, F, Simula, L, Marras, L, Piccolo, D, Cherchi, GB, Graham, DY & Realdi, G 2003, 'Detection of Chlamydiae pneumoniae but not Helicobacter pylori DNA in atherosclerosis plaques', Digestive Diseases and Sciences, vol. 48, no. 5, pp. 945-951. https://doi.org/10.1023/A:1023059815117
Dore, Maria Pina ; Sepulveda, Antonia R. ; Bacciu, Piero P. ; Blasi, Francesco ; Simula, Luigi ; Marras, Lea ; Piccolo, Davide ; Cherchi, Giovanni B. ; Graham, David Y. ; Realdi, Giuseppe. / Detection of Chlamydiae pneumoniae but not Helicobacter pylori DNA in atherosclerosis plaques. In: Digestive Diseases and Sciences. 2003 ; Vol. 48, No. 5. pp. 945-951.
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