Detection of circulating tumour cells in breast cancer patients using human mammaglobin RT-PCR: Association with clinical prognostic factors

Paola Ferro, Maria Cristiana Franceschini, Bartolomeo Bacigalupo, Paolo Dessanti, Emilio Falco, Vincenzo Fontana, Daniela Gianquinto, Maria Pia Pistillo, Franco Fedeli, Silvio Roncella

Research output: Contribution to journalArticle

Abstract

Background: So far discordant results regarding the significance of tumour cells circulating in peripheral blood (CTCs) of breast cancer (BC) patients have been reported. Our aim was to evaluate the association of indirect CTC detection by amplification of human mammaglobin (hMAM) gene expression with traditional prognostic markers of clinical outcome in BC at the time of diagnosis. Patients and Methods: Peripheral blood samples from 190 patients with invasive and 12 patients with in situ BC, before therapy and/or surgery, from 184 patients with benign breast disease and from 146 healthy volunteers were tested for hMAM expression by a nested reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Correlations between CTCs and age at diagnosis, tumour type and size, grading, lymph node involvement, oestrogen and progesterone receptor status, HER-2/neu expression and Ki-67/MIB-1 labelling index were assessed through the odds ratio (OR) point estimates, considering OR >2.0 or

Original languageEnglish
Pages (from-to)2377-2382
Number of pages6
JournalAnticancer Research
Volume30
Issue number6
Publication statusPublished - Jun 2010

Keywords

  • Breast cancer
  • Circulating tumour cells
  • Human mammaglobin
  • Reverse-transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Ferro, P., Franceschini, M. C., Bacigalupo, B., Dessanti, P., Falco, E., Fontana, V., Gianquinto, D., Pistillo, M. P., Fedeli, F., & Roncella, S. (2010). Detection of circulating tumour cells in breast cancer patients using human mammaglobin RT-PCR: Association with clinical prognostic factors. Anticancer Research, 30(6), 2377-2382.