Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: Correlation with clinical stage of disease

Angela Gradilone, Paola Gazzaniga, Ida Silvestri, Orietta Gandini, Luciana Trasatti, Salvatore Lauro, Luigi Frati, Anna Maria Aglianò

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Expression of genes such as cytokeratin 19 (CK19), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) has been investigated at mRNA level in peripheral blood of carcinoma patients to detect the presence of circulating tumor cells (CTC). We performed this study because recent literature emphasizes that the importance of CK 19, 20 and EGFR mRNAs in CTC as prognostic factors remains unclear especially for breast, head and neck and colon cancer patients. Reverse transcriptase polymerase chain reaction (RT-PCR) followed by Southern blot hybridization was performed in blood samples from 47 subjects (12 colorectal, 15 head and neck and 20 breast carcinoma patients), as well as in 35 healthy donors. The CK19 expression was found in 36/47 patients (9 colorectal, 9 head and neck and 18 breast cancer), two patients (one affected by colorectal and one by head and neck cancer) were positive for CK20 whereas EGFR was found expressed in 9 patients (3 colorectal, 5 head and neck and one breast cancer). Seven of 35 and 4/35 healthy donors displayed positivity for the expression of CK19 and CK20 genes respectively, whereas no EGFR mRNA was found in this group. The correlation of the detection of CTC in peripheral blood with progression of the disease in a follow-up period of 40 months did not show any prognostic value to the presence of mRNAs of these biomarkers in blood. We believe that research should be addressed, at least for breast cancer, to the identification of occult metastases in sentinel lymph nodes, such as recently performed in melanoma patients.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalOncology Reports
Volume10
Issue number1
Publication statusPublished - Jan 2003

Fingerprint

Keratin-20
Keratin-19
Epidermal Growth Factor Receptor
Carcinoma
Messenger RNA
Circulating Neoplastic Cells
Breast Neoplasms
Neck
Head
Head and Neck Neoplasms
Tissue Donors
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Colonic Neoplasms
Disease Progression
Melanoma
Breast
Biomarkers
Neoplasm Metastasis
Gene Expression

Keywords

  • Blood
  • Carcinoma
  • CK19
  • CK20
  • EGFR
  • RT-PCR

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Gradilone, A., Gazzaniga, P., Silvestri, I., Gandini, O., Trasatti, L., Lauro, S., ... Aglianò, A. M. (2003). Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: Correlation with clinical stage of disease. Oncology Reports, 10(1), 217-222.

Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients : Correlation with clinical stage of disease. / Gradilone, Angela; Gazzaniga, Paola; Silvestri, Ida; Gandini, Orietta; Trasatti, Luciana; Lauro, Salvatore; Frati, Luigi; Aglianò, Anna Maria.

In: Oncology Reports, Vol. 10, No. 1, 01.2003, p. 217-222.

Research output: Contribution to journalArticle

Gradilone, A, Gazzaniga, P, Silvestri, I, Gandini, O, Trasatti, L, Lauro, S, Frati, L & Aglianò, AM 2003, 'Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: Correlation with clinical stage of disease', Oncology Reports, vol. 10, no. 1, pp. 217-222.
Gradilone A, Gazzaniga P, Silvestri I, Gandini O, Trasatti L, Lauro S et al. Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: Correlation with clinical stage of disease. Oncology Reports. 2003 Jan;10(1):217-222.
Gradilone, Angela ; Gazzaniga, Paola ; Silvestri, Ida ; Gandini, Orietta ; Trasatti, Luciana ; Lauro, Salvatore ; Frati, Luigi ; Aglianò, Anna Maria. / Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients : Correlation with clinical stage of disease. In: Oncology Reports. 2003 ; Vol. 10, No. 1. pp. 217-222.
@article{2b4f1a3eb5e543e2af826a5063bc7041,
title = "Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: Correlation with clinical stage of disease",
abstract = "Expression of genes such as cytokeratin 19 (CK19), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) has been investigated at mRNA level in peripheral blood of carcinoma patients to detect the presence of circulating tumor cells (CTC). We performed this study because recent literature emphasizes that the importance of CK 19, 20 and EGFR mRNAs in CTC as prognostic factors remains unclear especially for breast, head and neck and colon cancer patients. Reverse transcriptase polymerase chain reaction (RT-PCR) followed by Southern blot hybridization was performed in blood samples from 47 subjects (12 colorectal, 15 head and neck and 20 breast carcinoma patients), as well as in 35 healthy donors. The CK19 expression was found in 36/47 patients (9 colorectal, 9 head and neck and 18 breast cancer), two patients (one affected by colorectal and one by head and neck cancer) were positive for CK20 whereas EGFR was found expressed in 9 patients (3 colorectal, 5 head and neck and one breast cancer). Seven of 35 and 4/35 healthy donors displayed positivity for the expression of CK19 and CK20 genes respectively, whereas no EGFR mRNA was found in this group. The correlation of the detection of CTC in peripheral blood with progression of the disease in a follow-up period of 40 months did not show any prognostic value to the presence of mRNAs of these biomarkers in blood. We believe that research should be addressed, at least for breast cancer, to the identification of occult metastases in sentinel lymph nodes, such as recently performed in melanoma patients.",
keywords = "Blood, Carcinoma, CK19, CK20, EGFR, RT-PCR",
author = "Angela Gradilone and Paola Gazzaniga and Ida Silvestri and Orietta Gandini and Luciana Trasatti and Salvatore Lauro and Luigi Frati and Aglian{\`o}, {Anna Maria}",
year = "2003",
month = "1",
language = "English",
volume = "10",
pages = "217--222",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients

T2 - Correlation with clinical stage of disease

AU - Gradilone, Angela

AU - Gazzaniga, Paola

AU - Silvestri, Ida

AU - Gandini, Orietta

AU - Trasatti, Luciana

AU - Lauro, Salvatore

AU - Frati, Luigi

AU - Aglianò, Anna Maria

PY - 2003/1

Y1 - 2003/1

N2 - Expression of genes such as cytokeratin 19 (CK19), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) has been investigated at mRNA level in peripheral blood of carcinoma patients to detect the presence of circulating tumor cells (CTC). We performed this study because recent literature emphasizes that the importance of CK 19, 20 and EGFR mRNAs in CTC as prognostic factors remains unclear especially for breast, head and neck and colon cancer patients. Reverse transcriptase polymerase chain reaction (RT-PCR) followed by Southern blot hybridization was performed in blood samples from 47 subjects (12 colorectal, 15 head and neck and 20 breast carcinoma patients), as well as in 35 healthy donors. The CK19 expression was found in 36/47 patients (9 colorectal, 9 head and neck and 18 breast cancer), two patients (one affected by colorectal and one by head and neck cancer) were positive for CK20 whereas EGFR was found expressed in 9 patients (3 colorectal, 5 head and neck and one breast cancer). Seven of 35 and 4/35 healthy donors displayed positivity for the expression of CK19 and CK20 genes respectively, whereas no EGFR mRNA was found in this group. The correlation of the detection of CTC in peripheral blood with progression of the disease in a follow-up period of 40 months did not show any prognostic value to the presence of mRNAs of these biomarkers in blood. We believe that research should be addressed, at least for breast cancer, to the identification of occult metastases in sentinel lymph nodes, such as recently performed in melanoma patients.

AB - Expression of genes such as cytokeratin 19 (CK19), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) has been investigated at mRNA level in peripheral blood of carcinoma patients to detect the presence of circulating tumor cells (CTC). We performed this study because recent literature emphasizes that the importance of CK 19, 20 and EGFR mRNAs in CTC as prognostic factors remains unclear especially for breast, head and neck and colon cancer patients. Reverse transcriptase polymerase chain reaction (RT-PCR) followed by Southern blot hybridization was performed in blood samples from 47 subjects (12 colorectal, 15 head and neck and 20 breast carcinoma patients), as well as in 35 healthy donors. The CK19 expression was found in 36/47 patients (9 colorectal, 9 head and neck and 18 breast cancer), two patients (one affected by colorectal and one by head and neck cancer) were positive for CK20 whereas EGFR was found expressed in 9 patients (3 colorectal, 5 head and neck and one breast cancer). Seven of 35 and 4/35 healthy donors displayed positivity for the expression of CK19 and CK20 genes respectively, whereas no EGFR mRNA was found in this group. The correlation of the detection of CTC in peripheral blood with progression of the disease in a follow-up period of 40 months did not show any prognostic value to the presence of mRNAs of these biomarkers in blood. We believe that research should be addressed, at least for breast cancer, to the identification of occult metastases in sentinel lymph nodes, such as recently performed in melanoma patients.

KW - Blood

KW - Carcinoma

KW - CK19

KW - CK20

KW - EGFR

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=0742281686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0742281686&partnerID=8YFLogxK

M3 - Article

C2 - 12469172

AN - SCOPUS:0742281686

VL - 10

SP - 217

EP - 222

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 1

ER -