Detection of cytotoxic T lymphocytes specific for synthetic peptides of gp160 in HIV-seropositive individuals

Mario Clerici, Daniel R. Lucey, Robert A. Zajac, R. Neal Boswell, Howard M. Gebel, Hidemi Takahashi, Jay A. Berzofsky, Gene M. Shearer

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Abstract

Four synthetic peptides corresponding to the IIIB sequence of gp160 of HIV were recently reported to stimulate Th cell function by PBL from HIV-infected, asymptomatic patients. In the present report, we used these same peptides to demonstrate CTL activity in a similar patient population. EBV-transformed B-cell lines from asymptomatic, HIV seropositive and seronegative control donors were pre-incubated with the peptides. Fresh PBL from 19 (76%) of 25 HIV seropositive donors lysed autologous targets pulsed with at least one of the four peptides. Autologous targets pulsed with two non-immunogenic peptides were not lysed. PBL from none of the eight HIV seronegative controls lysed peptide-preincubated autologous targets. The CTL activity was mediated by T cells, was predominantly MHC class I restricted, and was increased by in vitro restimulation of PBL with the peptides. HLA A-2 was identified as a restricting element for all four peptides in different patients, and for three of the peptides in the same donor. HLA-A1 or -B8 may also present some of the peptides. Thus, the same peptides can be recognized by human Th cells and class I MHC-restricted CTL.

Original languageEnglish
Pages (from-to)2214-2219
Number of pages6
JournalJournal of Immunology
Volume146
Issue number7
Publication statusPublished - Apr 1 1991

ASJC Scopus subject areas

  • Immunology

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    Clerici, M., Lucey, D. R., Zajac, R. A., Boswell, R. N., Gebel, H. M., Takahashi, H., Berzofsky, J. A., & Shearer, G. M. (1991). Detection of cytotoxic T lymphocytes specific for synthetic peptides of gp160 in HIV-seropositive individuals. Journal of Immunology, 146(7), 2214-2219.