Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

Silvia Vanzulli, Silvina Gazzaniga, María F. Braidot, Annunciata Vecchi, Alberto Mantovani, Rosa Wainstok De Calmanovici

Research output: Contribution to journalArticle

Abstract

In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalBiocell
Volume21
Issue number1
Publication statusPublished - 1997

Keywords

  • Endothelial cells
  • MEC 13.3
  • Mice mammary tumors
  • Mouse CD31/PECAM

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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    Vanzulli, S., Gazzaniga, S., Braidot, M. F., Vecchi, A., Mantovani, A., & Wainstok De Calmanovici, R. (1997). Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell, 21(1), 39-46.