Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

Silvia Vanzulli, Silvina Gazzaniga, María F. Braidot, Annunciata Vecchi, Alberto Mantovani, Rosa Wainstok De Calmanovici

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalBiocell
Volume21
Issue number1
Publication statusPublished - 1997

Fingerprint

Endothelial Cells
Monoclonal Antibodies
Breast Neoplasms
Blood Vessels
Staining and Labeling
Neoplasms
Vascular Endothelium
Frozen Sections
Theoretical Models
Membranes
Research

Keywords

  • Endothelial cells
  • MEC 13.3
  • Mice mammary tumors
  • Mouse CD31/PECAM

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Vanzulli, S., Gazzaniga, S., Braidot, M. F., Vecchi, A., Mantovani, A., & Wainstok De Calmanovici, R. (1997). Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell, 21(1), 39-46.

Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. / Vanzulli, Silvia; Gazzaniga, Silvina; Braidot, María F.; Vecchi, Annunciata; Mantovani, Alberto; Wainstok De Calmanovici, Rosa.

In: Biocell, Vol. 21, No. 1, 1997, p. 39-46.

Research output: Contribution to journalArticle

Vanzulli, S, Gazzaniga, S, Braidot, MF, Vecchi, A, Mantovani, A & Wainstok De Calmanovici, R 1997, 'Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors', Biocell, vol. 21, no. 1, pp. 39-46.
Vanzulli S, Gazzaniga S, Braidot MF, Vecchi A, Mantovani A, Wainstok De Calmanovici R. Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell. 1997;21(1):39-46.
Vanzulli, Silvia ; Gazzaniga, Silvina ; Braidot, María F. ; Vecchi, Annunciata ; Mantovani, Alberto ; Wainstok De Calmanovici, Rosa. / Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. In: Biocell. 1997 ; Vol. 21, No. 1. pp. 39-46.
@article{17f4572922954d95bd8ab9bc0464d853,
title = "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors",
abstract = "In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.",
keywords = "Endothelial cells, MEC 13.3, Mice mammary tumors, Mouse CD31/PECAM",
author = "Silvia Vanzulli and Silvina Gazzaniga and Braidot, {Mar{\'i}a F.} and Annunciata Vecchi and Alberto Mantovani and {Wainstok De Calmanovici}, Rosa",
year = "1997",
language = "English",
volume = "21",
pages = "39--46",
journal = "Biocell",
issn = "0327-9545",
publisher = "Universidad Nacional de Cuyo",
number = "1",

}

TY - JOUR

T1 - Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

AU - Vanzulli, Silvia

AU - Gazzaniga, Silvina

AU - Braidot, María F.

AU - Vecchi, Annunciata

AU - Mantovani, Alberto

AU - Wainstok De Calmanovici, Rosa

PY - 1997

Y1 - 1997

N2 - In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.

AB - In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.

KW - Endothelial cells

KW - MEC 13.3

KW - Mice mammary tumors

KW - Mouse CD31/PECAM

UR - http://www.scopus.com/inward/record.url?scp=0031111610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031111610&partnerID=8YFLogxK

M3 - Article

C2 - 9212717

AN - SCOPUS:0031111610

VL - 21

SP - 39

EP - 46

JO - Biocell

JF - Biocell

SN - 0327-9545

IS - 1

ER -