Detection of erythroblast antibodies in mitogen-stimulated bone marrow cultures from patients with myelodysplastic syndromes

Anna Zaninoni, Francesca G. Imperiali, Alessandra Cattaneo, Giulia Soverini, Francesca Binda, Laura Porretti, Agostino Cortelezzi, Wilma Barcellini

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Low-risk myelodysplastic syndromes (MDS) show several immunologic abnormalities, including increased frequency of autoimmune manifestations and/or overt autoimmune diseases, whose prognostic significance still remains controversial. STUDY DESIGN AND METHODS: We studied the presence of erythroblast antibodies in mitogen-stimulated bone marrow (BM) cultures of 70 patients with early-stage MDS (refractory anemia and refractory anemia with ringed sideroblasts). RESULTS: Sixty-six percent of patients showed positive erythroblast antibodies, along with BM erythroid hyperplasia and a hemolytic picture in the peripheral blood. Supernatants from positive cultures induced an increase of overall cellularity, the appearance of erythroblastic clustering, and dyserythropoietic signs in normal BM. We identified CD45dimGly-AdimCD71bright cells (red blood cell precursors at different maturation stage) as the target of the antibodies. Erythropoietin (EPO) levels were reduced and EPO receptors (EPO-R) increased in BM culture supernatants from positive patients. However, flow cytometric analysis showed that neither EPO nor EPO-R was involved in an abnormal stimulation driven by these autoantibodies. Values of the proapoptotic protein Bax were increased in positive patients and Bcl-2 levels were decreased, although not significantly. CONCLUSION: MDS patients with anti-erythroblast autoimmunity showed increased BM apoptosis, suggesting that the autoimmune reaction may contribute to an unfavorable BM microenvironment for optimal erythropoiesis.

Original languageEnglish
Pages (from-to)2037-2041
Number of pages5
JournalTransfusion
Volume56
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

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Erythroblasts
Myelodysplastic Syndromes
Mitogens
Bone Marrow
Antibodies
Erythropoietin
Refractory Anemia
Erythropoietin Receptors
bcl-2-Associated X Protein
Erythropoiesis
Autoimmunity
Autoantibodies
Autoimmune Diseases
Hyperplasia
Cluster Analysis
Erythrocytes
Apoptosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Medicine(all)
  • Hematology

Cite this

Detection of erythroblast antibodies in mitogen-stimulated bone marrow cultures from patients with myelodysplastic syndromes. / Zaninoni, Anna; Imperiali, Francesca G.; Cattaneo, Alessandra; Soverini, Giulia; Binda, Francesca; Porretti, Laura; Cortelezzi, Agostino; Barcellini, Wilma.

In: Transfusion, Vol. 56, No. 8, 01.08.2016, p. 2037-2041.

Research output: Contribution to journalArticle

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