Detection of fibronectin-binding protein genes in staphylococcal strains from peri-prosthesis infections

L. Montanaro, C. R. Arciola, E. Borsetti, S. Collamati, L. Baldassarri, L. Montanaro

Research output: Contribution to journalArticle

Abstract

Several studies have been devoted to identify the adhesion mechanisms of Staphylococcus aureus and Staphylococcus epidermidis, which are the most frequent causes of prosthesis-associated infections. Recently, in particular for Staphylococcus aureus, considerable attention has been given to the host protein receptors as mediators for bacterial adherence. Fibronectin, an important matrix protein, seems to be a major ligand for bacterial adherence in the early stages of infection. To determine the importance of the fibronectin adhesin as virulence factor in Staphylococcus-induced prosthesis infection, a simple and reliable method using a polymerase chain reaction (PCR) was devised to identify fibronectin adhesin genes (fnbA and fnbB). Results obtained by this method were in accordance with those obtained by the in vitro phenotypic characterization of binding ability to fibronectin of Staphylococcus strains.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalNew Microbiologica
Volume22
Issue number4
Publication statusPublished - Oct 1999

Keywords

  • Bacterial adhesion
  • Biomaterial-associated infection
  • Fibronectin-binding protein gene (fnbA, fbnB)
  • Polymerase chain reaction (PCR)
  • Staphylococcus aureus
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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  • Cite this

    Montanaro, L., Arciola, C. R., Borsetti, E., Collamati, S., Baldassarri, L., & Montanaro, L. (1999). Detection of fibronectin-binding protein genes in staphylococcal strains from peri-prosthesis infections. New Microbiologica, 22(4), 331-336.