Detection of HBV infectivity by spot hybridization in HBeAg-negative chronic carriers: HBV DNA in sera from asymptomatic and symptomatic subjects

G. Carloni, S. Colloca, C. Delfini, A. Manzin, M. Clementi, F. Galibert

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Abstract

DNA of hepatitis B virus (HBV DNA) in sera from HBeAg-positive carriers is now the most important and reliable marker of infectivity, but its significance in the progression of chronic hepatitis in anti-HBe carrier status is still under discussion. In this study, viral DNA was tested by a simplified spot hybridization method in sera of 206 HBeAg-negative Italian subjects. In a group of 153 HBsAg carriers, we found that 15.6% of anti-HBe-positive and 10.5% of anti-HBe-negative samples contained viral DNA. No HBV DNA was revealed in 38 HBsAg-negative nor in 15 anti-HBs-positive subjects with different serological markers of HBV. Viral DNA in sera of HBeAg-negative patients with severe chronic liver disease was correlated with increased alaninetransferase activity and IgM anti-HBc. Thus the presence of HBV DNA in these sera not only predicts which subjects are potentially infectious but also indicates chronic progression of hepatitis. Finally, viral DNA extracted from Dane particles of nine anti-HBe-positive sera was characterized by the Southern blot technique. The hybridization pattern shows bands indicating the presence of replicative intermediates.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalJournal of Medical Virology
Volume21
Issue number1
DOIs
Publication statusPublished - 1987

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Hepatitis B e Antigens
Viral DNA
DNA
Serum
Chronic Hepatitis
Hepatitis B Surface Antigens
Hepatitis B virus
Southern Blotting
Liver Diseases
Chronic Disease

ASJC Scopus subject areas

  • Virology

Cite this

Detection of HBV infectivity by spot hybridization in HBeAg-negative chronic carriers : HBV DNA in sera from asymptomatic and symptomatic subjects. / Carloni, G.; Colloca, S.; Delfini, C.; Manzin, A.; Clementi, M.; Galibert, F.

In: Journal of Medical Virology, Vol. 21, No. 1, 1987, p. 15-23.

Research output: Contribution to journalArticle

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AU - Galibert, F.

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