Detection of hepatitis a virus and other enteric viruses in shellfish collected in the gulf of naples, italy

Giovanna Fusco, Aniello Anastasio, David H. Kingsley, Maria Grazia Amoroso, Tiziana Pepe, Pina M. Fratamico, Barbara Cioffi, Rachele Rossi, Giuseppina La Rosa, Federica Boccia

Research output: Contribution to journalArticlepeer-review

Abstract

To assess the quality of shellfish harvest areas, bivalve mollusk samples from three coastal areas of the Campania region in Southwest Italy were evaluated for viruses over a three-year period (2015–2017). Screening of 289 samples from shellfish farms and other locations by qPCR and RTqPCR identified hepatitis A virus (HAV; 8.9%), norovirus GI (NoVGI; 10.8%) and GII (NoVGII; 39.7%), rotavirus (RV; 9.0%), astrovirus (AsV; 20.8%), sapovirus (SaV; 18.8%), aichivirus-1 (AiV-1; 5.6%), and adenovirus (AdV, 5.6%). Hepatitis E virus (HEV) was never detected. Sequence analysis identified HAV as genotype IA and AdV as type 41. This study demonstrates the presence of different enteric viruses within bivalve mollusks, highlighting the limitations of the current EU classification system for shellfish growing waters.

Original languageEnglish
Article number2588
JournalInternational Journal of Environmental Research and Public Health
Volume16
Issue number14
DOIs
Publication statusPublished - Jul 2 2019

Keywords

  • Enteric viruses
  • HAV
  • Molecular methods
  • Mollusks

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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