Abstract
The presence of HCV RNA in peripheral blood mononuclear cells (PBMC) has been reported. To identify the cell populations carrying HCV RNA, the presence and amount of HCV RNA was investigated by limiting dilution nested reverse transcriptase-polymerase chain reaction (PCR) in PBMC subpopulations fractionated by automated cell sorting. Fifteen chronically HCV-infected patients were included in the study, 4 of whom also had mixed cryoglobulinemia. HCV RNA was present in the CD19 cells of all 15 patients, but only 5 (35.7%) of 14 and 5 (41.6%) of 12 showed HCV RNA in CD3 and CD14 cells, respectively (P <.001 by Fisher's test for each comparison). The median titer of HCV RNA was 1 PCR unit/380 CD19 cells, compared with median of 1 PCR unit/6600 PBMC as a whole. Titration was difficult in the CD3 and CD14 cells because of the frequent negativity of the first diluted sample. This study suggests that HCV RNA is selectively concentrated in B cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1209-1214 |
| Number of pages | 6 |
| Journal | Journal of Infectious Diseases |
| Volume | 176 |
| Issue number | 5 |
| Publication status | Published - 1997 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Public Health, Environmental and Occupational Health
Cite this
Detection of hepatitis C virus RNA in CD19 peripheral blood mononuclear cells of chronically infected patients. / Zehender, Gianguglielmo; Meroni, Luca; De Maddalena, Chiara; Varchetta, Stefania; Monti, Giuseppe; Galli, Massimo.
In: Journal of Infectious Diseases, Vol. 176, No. 5, 1997, p. 1209-1214.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of hepatitis C virus RNA in CD19 peripheral blood mononuclear cells of chronically infected patients
AU - Zehender, Gianguglielmo
AU - Meroni, Luca
AU - De Maddalena, Chiara
AU - Varchetta, Stefania
AU - Monti, Giuseppe
AU - Galli, Massimo
PY - 1997
Y1 - 1997
N2 - The presence of HCV RNA in peripheral blood mononuclear cells (PBMC) has been reported. To identify the cell populations carrying HCV RNA, the presence and amount of HCV RNA was investigated by limiting dilution nested reverse transcriptase-polymerase chain reaction (PCR) in PBMC subpopulations fractionated by automated cell sorting. Fifteen chronically HCV-infected patients were included in the study, 4 of whom also had mixed cryoglobulinemia. HCV RNA was present in the CD19 cells of all 15 patients, but only 5 (35.7%) of 14 and 5 (41.6%) of 12 showed HCV RNA in CD3 and CD14 cells, respectively (P <.001 by Fisher's test for each comparison). The median titer of HCV RNA was 1 PCR unit/380 CD19 cells, compared with median of 1 PCR unit/6600 PBMC as a whole. Titration was difficult in the CD3 and CD14 cells because of the frequent negativity of the first diluted sample. This study suggests that HCV RNA is selectively concentrated in B cells.
AB - The presence of HCV RNA in peripheral blood mononuclear cells (PBMC) has been reported. To identify the cell populations carrying HCV RNA, the presence and amount of HCV RNA was investigated by limiting dilution nested reverse transcriptase-polymerase chain reaction (PCR) in PBMC subpopulations fractionated by automated cell sorting. Fifteen chronically HCV-infected patients were included in the study, 4 of whom also had mixed cryoglobulinemia. HCV RNA was present in the CD19 cells of all 15 patients, but only 5 (35.7%) of 14 and 5 (41.6%) of 12 showed HCV RNA in CD3 and CD14 cells, respectively (P <.001 by Fisher's test for each comparison). The median titer of HCV RNA was 1 PCR unit/380 CD19 cells, compared with median of 1 PCR unit/6600 PBMC as a whole. Titration was difficult in the CD3 and CD14 cells because of the frequent negativity of the first diluted sample. This study suggests that HCV RNA is selectively concentrated in B cells.
UR - http://www.scopus.com/inward/record.url?scp=0030830876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030830876&partnerID=8YFLogxK
M3 - Article
C2 - 9359720
AN - SCOPUS:0030830876
VL - 176
SP - 1209
EP - 1214
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -