Detection of hepatitis C virus RNA in CD19 peripheral blood mononuclear cells of chronically infected patients

Gianguglielmo Zehender, Luca Meroni, Chiara De Maddalena, Stefania Varchetta, Giuseppe Monti, Massimo Galli

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The presence of HCV RNA in peripheral blood mononuclear cells (PBMC) has been reported. To identify the cell populations carrying HCV RNA, the presence and amount of HCV RNA was investigated by limiting dilution nested reverse transcriptase-polymerase chain reaction (PCR) in PBMC subpopulations fractionated by automated cell sorting. Fifteen chronically HCV-infected patients were included in the study, 4 of whom also had mixed cryoglobulinemia. HCV RNA was present in the CD19 cells of all 15 patients, but only 5 (35.7%) of 14 and 5 (41.6%) of 12 showed HCV RNA in CD3 and CD14 cells, respectively (P <.001 by Fisher's test for each comparison). The median titer of HCV RNA was 1 PCR unit/380 CD19 cells, compared with median of 1 PCR unit/6600 PBMC as a whole. Titration was difficult in the CD3 and CD14 cells because of the frequent negativity of the first diluted sample. This study suggests that HCV RNA is selectively concentrated in B cells.

Original languageEnglish
Pages (from-to)1209-1214
Number of pages6
JournalJournal of Infectious Diseases
Volume176
Issue number5
Publication statusPublished - 1997

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Hepacivirus
Blood Cells
RNA
Cryoglobulinemia
Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
B-Lymphocytes
Population

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Detection of hepatitis C virus RNA in CD19 peripheral blood mononuclear cells of chronically infected patients. / Zehender, Gianguglielmo; Meroni, Luca; De Maddalena, Chiara; Varchetta, Stefania; Monti, Giuseppe; Galli, Massimo.

In: Journal of Infectious Diseases, Vol. 176, No. 5, 1997, p. 1209-1214.

Research output: Contribution to journalArticle

Zehender, G, Meroni, L, De Maddalena, C, Varchetta, S, Monti, G & Galli, M 1997, 'Detection of hepatitis C virus RNA in CD19 peripheral blood mononuclear cells of chronically infected patients', Journal of Infectious Diseases, vol. 176, no. 5, pp. 1209-1214.
Zehender, Gianguglielmo ; Meroni, Luca ; De Maddalena, Chiara ; Varchetta, Stefania ; Monti, Giuseppe ; Galli, Massimo. / Detection of hepatitis C virus RNA in CD19 peripheral blood mononuclear cells of chronically infected patients. In: Journal of Infectious Diseases. 1997 ; Vol. 176, No. 5. pp. 1209-1214.
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