Detection of high molecular weight proteins by MALDI imaging mass spectrometry

Veronica Mainini, Giorgio Bovo, Clizia Chinello, Erica Gianazza, Marco Grasso, Giorgio Cattoretti, Fulvio Magni

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.

Original languageEnglish
Pages (from-to)1101-1107
Number of pages7
JournalMolecular BioSystems
Volume9
Issue number6
DOIs
Publication statusPublished - 2013

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass Spectrometry
ferulic acid
Molecular Weight
Neuropeptide Receptors
Technology
Biological Phenomena
Enzyme Precursors
Proteins
Cytokines
Peptides

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Medicine(all)

Cite this

Mainini, V., Bovo, G., Chinello, C., Gianazza, E., Grasso, M., Cattoretti, G., & Magni, F. (2013). Detection of high molecular weight proteins by MALDI imaging mass spectrometry. Molecular BioSystems, 9(6), 1101-1107. https://doi.org/10.1039/c2mb25296a

Detection of high molecular weight proteins by MALDI imaging mass spectrometry. / Mainini, Veronica; Bovo, Giorgio; Chinello, Clizia; Gianazza, Erica; Grasso, Marco; Cattoretti, Giorgio; Magni, Fulvio.

In: Molecular BioSystems, Vol. 9, No. 6, 2013, p. 1101-1107.

Research output: Contribution to journalArticle

Mainini, V, Bovo, G, Chinello, C, Gianazza, E, Grasso, M, Cattoretti, G & Magni, F 2013, 'Detection of high molecular weight proteins by MALDI imaging mass spectrometry', Molecular BioSystems, vol. 9, no. 6, pp. 1101-1107. https://doi.org/10.1039/c2mb25296a
Mainini, Veronica ; Bovo, Giorgio ; Chinello, Clizia ; Gianazza, Erica ; Grasso, Marco ; Cattoretti, Giorgio ; Magni, Fulvio. / Detection of high molecular weight proteins by MALDI imaging mass spectrometry. In: Molecular BioSystems. 2013 ; Vol. 9, No. 6. pp. 1101-1107.
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