Detection of HIV-1 in epidermal Langerhans cells of HIV-infected patients using the polymerase chain reaction

G. Zambruno, L. Mori, A. Marconi, N. Mongiardo, B. De Rienzo, U. Bertazzoni, A. Giannetti

Research output: Contribution to journalArticle

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Abstract

Langerhans cells (LC) are bone marrow-derived, HLA-DR+, CD1a+, dendritic antigen-presenting cells found in stratified squamous epithelia. Within resident epidermal cells (EC), LC are the only cells expressing the CD4 antigen and are, therefore, a possible target for human immunodeficiency virus (HIV) infection. To data, conflicting results have been reported on the in vivo infection of LC by HIV. The aim of the present study was to investigate the presence of HIV-1 proviral DNA in epidermal LC of HIV-1-infected patients. EC suspensions were prepared from clinically normal skin of nine seropositive patients. Purified LC and LC-depleted EC were obtained by immunomagnetic separation and analyzed for the presence of HIV-1 proviral DNA by the polymerase chain reaction using primer pairs from different conserved regions (env and gag) of the HIV-1 genome. HIV-1 proviral DNA was detected in LC from seven of nine patients. LC-depleted EC fractions from the same nine patients were all negative, with the exception of one case. Altogether these results demonstrate that epidermal LC are infected by HIV-1 and constitute the only resident cell type in the epidermis harboring the virus. Further studies are, however, needed to demonstrate HIV replication in LC and to elucidate the functional role of LC in this infection.

Original languageEnglish
Pages (from-to)979-982
Number of pages4
JournalJournal of Investigative Dermatology
Volume96
Issue number6
Publication statusPublished - 1991

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Langerhans Cells
Polymerase chain reaction
Viruses
HIV-1
HIV
Polymerase Chain Reaction
Immunomagnetic Separation
CD4 Antigens
DNA
HLA-DR Antigens
DNA-Directed DNA Polymerase
Antigen-Presenting Cells
Virus Diseases
Virus Replication
Infection
Epidermis
Dendritic Cells
Suspensions
Skin
Bone

ASJC Scopus subject areas

  • Dermatology

Cite this

Zambruno, G., Mori, L., Marconi, A., Mongiardo, N., De Rienzo, B., Bertazzoni, U., & Giannetti, A. (1991). Detection of HIV-1 in epidermal Langerhans cells of HIV-infected patients using the polymerase chain reaction. Journal of Investigative Dermatology, 96(6), 979-982.

Detection of HIV-1 in epidermal Langerhans cells of HIV-infected patients using the polymerase chain reaction. / Zambruno, G.; Mori, L.; Marconi, A.; Mongiardo, N.; De Rienzo, B.; Bertazzoni, U.; Giannetti, A.

In: Journal of Investigative Dermatology, Vol. 96, No. 6, 1991, p. 979-982.

Research output: Contribution to journalArticle

Zambruno, G, Mori, L, Marconi, A, Mongiardo, N, De Rienzo, B, Bertazzoni, U & Giannetti, A 1991, 'Detection of HIV-1 in epidermal Langerhans cells of HIV-infected patients using the polymerase chain reaction', Journal of Investigative Dermatology, vol. 96, no. 6, pp. 979-982.
Zambruno, G. ; Mori, L. ; Marconi, A. ; Mongiardo, N. ; De Rienzo, B. ; Bertazzoni, U. ; Giannetti, A. / Detection of HIV-1 in epidermal Langerhans cells of HIV-infected patients using the polymerase chain reaction. In: Journal of Investigative Dermatology. 1991 ; Vol. 96, No. 6. pp. 979-982.
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