Detection of HPV16/18 E6 Oncoproteins in Head and Neck Squamous Cell Carcinoma Using a Protein Immunochromatographic Assay

Anna Menegaldo, Lea Schroeder, Dana Holzinger, Giancarlo Tirelli, Elisa Dal Cin, Margherita Tofanelli, Stefania Rigo, Monica Mantovani, Marco Stellin, Annarosa Del Mistro, Angelo P. Dei Tos, Angela Guerriero, Monia Niero, Daniele Borsetto, Maria C. Da Mosto, Jerry Polesel, Michael Pawlita, Tim Waterboer, Paolo Boscolo-Rizzo

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives/Hypothesis: The accurate diagnostic assessment of clinically relevant human papillomavirus (HPV) infections in patients with head and neck squamous cell carcinoma represents an urgent unmet medical need. The aim of this study was to determine feasibility, accuracy, and clinical significance of HPV16/18 E6 oncoprotein detection on cytological specimens from oropharyngeal squamous cell carcinoma (OPSCC) and neck lymph node metastasis of SCC from unknown primary tumor (CUP) via a protein immunochromatographic assay. Study Design: Cross-sectional study. Methods: Cytological specimens from primary tumor and neck metastases were collected from 34 patients with OPSCC or CUP and applied to a lateral flow format test that detects HPV16 and HPV18 E6 oncoproteins. E6 oncoprotein positivity or negativity in these specimens was compared to the specimens' “HPV-driven” reference status, defined by presence of HPV-DNA in combination with p16INK4a overexpression and/or HPV E6 seropositivity. Results: Eighteen of 29 OPSCC (62%) and three of five CUP (60%) were HPV-driven according to our reference method. The E6 oncoprotein lateral flow test had a sensitivity of 94% (95% CI: 70%–100%) and a specificity of 100% (95% CI: 66%–100%) on primary tumor, and a sensitivity of 88% (95% CI: 64%–99%) and a specificity of 100% (95% CI: 74%–100%) on neck metastases. Test agreement between the E6 lateral flow test and the clinical reference method, HPV-DNA plus p16INK4a was excellent, both for primary lesion and neck metastases. Conclusions: We found the detection of HPV16/18 E6 oncoproteins to be a feasible, highly reliable, and low-invasive method to assess “HPV-driven” status in OPSCC and CUP. Level of Evidence: II Laryngoscope, 2020.

Original languageEnglish
JournalLaryngoscope
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • carcinoma of unknown primary
  • E6 oncoprotein
  • human papillomavirus
  • neck metastasis
  • Oropharyngeal squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

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