Detection of human herpesvirus 8 in cervicovaginal secretions and seroprevalence in human immunodeficiency virus type 1-seropositive and - seronegative women

Maria Luisa Calabrò, Josè Ramon Fiore, Anna Favero, Achiropita Lepera, Annalisa Saracino, Gioacchino Angarano, Thomas Friedrich Schulz, Luigi Chieco-Bianchi

Research output: Contribution to journalArticle

Abstract

Epidemiologic studies suggest that human herpesvirus 8 (HHV-8) may be sexually transmitted. To study the potential for HHV-8 transmission through cervicovaginal (CV) secretions, the presence of HHV-8 DNA was investigated by nested polymerase chain reaction in the cellular fraction of CV secretions from 36 human immunodeficiency virus type 1 (HIV-1)-seropositive and 29 HIV- 1-seronegative women. The same patients were tested for antibodies to two defined HHV-8 antigens (latency-associated nuclear antigen and open-reading frame 65-encoded structural protein) and for HHV-8 DNA in their peripheral blood mononuclear cells (PBMC). The findings were compared with the rate of HHV-8 detection in semen samples of 20 HIV-1-infected men. HHV-8 DNA was detected in the CV samples from only 1 HHV-8-seropositive AIDS patient, in 3 PBMC samples (1/29 HIV-1-seronegative patients, 1/3 AIDS patients with Kaposi's sarcoma, and 1/19 AIDS patients), and in 1 of 20 semen samples. HHV8 infection was more common in HIV-1-infected than uninfected women. Thus HHV- 8 DNA is only rarely detectable in CV secretions and semen of HHV-8-infected individuals.

Original languageEnglish
Pages (from-to)1534-1537
Number of pages4
JournalJournal of Infectious Diseases
Volume179
Issue number6
DOIs
Publication statusPublished - 1999

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ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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