Detection of human immunodeficiency virus type 1 genomic RNA in plasma samples by reverse-transcription polymerase chain reaction

P. Bagnarelli, S. Menzo, A. Manzin, M. Giacca, P. E. Varaldo, M. Clementi

Research output: Contribution to journalArticle

Abstract

An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Medical Virology
Volume34
Issue number2
Publication statusPublished - 1991

Keywords

  • Human immunodeficiency virus type 1
  • Polymerase chain reaction
  • Viremia

ASJC Scopus subject areas

  • Virology

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