TY - JOUR
T1 - Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction
AU - Bagnarelli, Patrizia
AU - Menzo, Stefano
AU - Manzin, Aldo
AU - Varaldo, Pietro E.
AU - Montroni, Maria
AU - Giacca, Mauro
AU - Clementi, Massimo
PY - 1991
Y1 - 1991
N2 - A simplified application of the polymerase chain reaction (PCR) to the routine detection of human immunodeficiency virus type 1 (HIV-1) transcripts from peripheral lymphocytes of infected subjects is described. This technique is simpler than previously described assays and was shown to be highly sensitive after ethidium bromide staining of polyacrylamide gel electrophoresis of amplified material. The method can be used for the virologie evaluation of HIV-1-infected subjects, thus allowing early identification of seropositive patients with signs of active infection.
AB - A simplified application of the polymerase chain reaction (PCR) to the routine detection of human immunodeficiency virus type 1 (HIV-1) transcripts from peripheral lymphocytes of infected subjects is described. This technique is simpler than previously described assays and was shown to be highly sensitive after ethidium bromide staining of polyacrylamide gel electrophoresis of amplified material. The method can be used for the virologie evaluation of HIV-1-infected subjects, thus allowing early identification of seropositive patients with signs of active infection.
KW - HIV-1
KW - Polymerase chain reaction
KW - Viral transcript
UR - http://www.scopus.com/inward/record.url?scp=0025730619&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025730619&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(91)90182-Y
DO - 10.1016/0166-0934(91)90182-Y
M3 - Article
C2 - 1712362
AN - SCOPUS:0025730619
VL - 32
SP - 31
EP - 39
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 1
ER -