Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction

Patrizia Bagnarelli, Stefano Menzo, Aldo Manzin, Pietro E. Varaldo, Maria Montroni, Mauro Giacca, Massimo Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

A simplified application of the polymerase chain reaction (PCR) to the routine detection of human immunodeficiency virus type 1 (HIV-1) transcripts from peripheral lymphocytes of infected subjects is described. This technique is simpler than previously described assays and was shown to be highly sensitive after ethidium bromide staining of polyacrylamide gel electrophoresis of amplified material. The method can be used for the virologie evaluation of HIV-1-infected subjects, thus allowing early identification of seropositive patients with signs of active infection.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalJournal of Virological Methods
Volume32
Issue number1
DOIs
Publication statusPublished - 1991

Keywords

  • HIV-1
  • Polymerase chain reaction
  • Viral transcript

ASJC Scopus subject areas

  • Virology

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