Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction

Patrizia Bagnarelli; Stefano Menzo; Aldo Manzin; Pietro E. Varaldo; Maria Montroni; Mauro Giacca; Massimo Clementi

doi:10.1016/0166-0934(91)90182-Y

Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction

Patrizia Bagnarelli, Stefano Menzo, Aldo Manzin, Pietro E. Varaldo, Maria Montroni, Mauro Giacca, Massimo Clementi

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

A simplified application of the polymerase chain reaction (PCR) to the routine detection of human immunodeficiency virus type 1 (HIV-1) transcripts from peripheral lymphocytes of infected subjects is described. This technique is simpler than previously described assays and was shown to be highly sensitive after ethidium bromide staining of polyacrylamide gel electrophoresis of amplified material. The method can be used for the virologie evaluation of HIV-1-infected subjects, thus allowing early identification of seropositive patients with signs of active infection.

Original language	English
Pages (from-to)	31-39
Number of pages	9
Journal	Journal of Virological Methods
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(91)90182-Y
Publication status	Published - 1991

Keywords

HIV-1
Polymerase chain reaction
Viral transcript

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(91)90182-Y

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Detection of human immunodeficiency virus type 1 transcripts in peripheral blood lymphocytes by the polymerase chain reaction. / Bagnarelli, Patrizia; Menzo, Stefano; Manzin, Aldo; Varaldo, Pietro E.; Montroni, Maria; Giacca, Mauro; Clementi, Massimo.

In: Journal of Virological Methods, Vol. 32, No. 1, 1991, p. 31-39.

Research output: Contribution to journal › Article › peer-review

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AU - Giacca, Mauro

AU - Clementi, Massimo

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