Detection of human mononuclear cell subsets by complement fixing monoclonal antibodies: Evaluation of manual procedures

P. Borzini, A. Aniasi, M. DeGiacomo, L. Bersani, G. Sirchia

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Abstract

Manual procedures to evaluate mononuclear cells reacted with monoclonal antibodies were evaluated with respect to reproducibility, ease of execution, and cost. The immunofluorescent technique and the complement-mediated cytotoxicity test produced very similar results; however, the latter was clearly preferable in terms of cost, time-consumption, and ease of execution. Evidence is also presented that purification of lymphocytes using gradient diffusion techniques may induce an uneven selectivity within the relative percentages of T cell subsets collected for analysis.

Original languageEnglish
JournalJournal of Clinical Immunology
Volume2
Issue numberSuppl. 3
Publication statusPublished - 1982

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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