Detection of leukotrienes B4, C4 and of their isomers in arterial, mixed venous blood and bronchoalveolar lavage fluid from ARDS patients

M. Antonelli, M. Bufi, R. A. De Blasi, G. Crimi, G. Conti, C. Mattia, G. Vivino, L. Lenti, D. Lombardi, A. Dotta, G. Pontieri, A. Gasparetto

Research output: Contribution to journalArticle

Abstract

Seven patients with the adult respiratory distress syndrome (ARDS) were studied. As a control group we used 6 surgical patients who underwent minor surgical operation (inguinal hernia). For both groups the same sample collection and analysis was used. The presence of leuktorienes (LTs) B4 and C4 and of their isomers 11-trans LTC4 and Δ6-trans-12-epi LTB4 was determined in arterial, mixed venous blood and in bronchoalveolar lavage (BAL) fluid. The samples, analysed by reverse phase high performance liquid chromatography (RP-HPLC), showed a similar chromatographic picture among ARDS patients, while the control group showed no detectable amounts of LTs in BAL or blood. The distribution of these arachidonic acid metabolites in mixed venous blood, arterial blood and BAL seems to suggest pulmonary metabolism and/or inactivation. It is suggested that these mediators act as humoral factors in pathogenesis of the ARDS.

Original languageEnglish
Pages (from-to)296-301
Number of pages6
JournalIntensive Care Medicine
Volume15
Issue number5
DOIs
Publication statusPublished - Jul 1989

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Keywords

  • ARDS
  • Humoral mediators
  • Leukotrienes

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Antonelli, M., Bufi, M., De Blasi, R. A., Crimi, G., Conti, G., Mattia, C., Vivino, G., Lenti, L., Lombardi, D., Dotta, A., Pontieri, G., & Gasparetto, A. (1989). Detection of leukotrienes B4, C4 and of their isomers in arterial, mixed venous blood and bronchoalveolar lavage fluid from ARDS patients. Intensive Care Medicine, 15(5), 296-301. https://doi.org/10.1007/BF00263864