Detection of molecules related to the GABAergic system in a single-cell eukaryote, Paramecium primaurelia

Maria U. Delmonte Corrado, Marzia Ognibene, Francesca Trielli, Huguette Politi, Mario Passalacqua, Carla Falugi

Research output: Contribution to journalArticle

Abstract

γ-Aminobutyric acid (GABA)-related molecules were identified in Paramecium primaurelia by immunocytochemical methods, and GABAA receptors by their histochemical BODIPY-binding sites. Confocal microscope analysis showed different localizations according to the stages of the developmental cycle. A comparison was made with the cholinergic molecules, such as the acetylcholine biosynthetic enzyme (choline acetyltransferase), in double-labelled cells by confocal microscopy. In vivo experiments suggested the involvement of GABA-related molecules in cell-cell interaction.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalNeuroscience Letters
Volume329
Issue number1
DOIs
Publication statusPublished - Aug 23 2002

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Paramecium
Eukaryota
gamma-Aminobutyric Acid
Aminobutyrates
Choline O-Acetyltransferase
GABA-A Receptors
Confocal Microscopy
Cell Communication
Cholinergic Agents
Acetylcholine
Binding Sites
Enzymes
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

Keywords

  • γ-Aminobutyric acid
  • γ-Aminobutyric acid receptor
  • Acetylcholine
  • Cell-cell interaction
  • Choline acetyltransferase
  • GABAse activity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Detection of molecules related to the GABAergic system in a single-cell eukaryote, Paramecium primaurelia. / Delmonte Corrado, Maria U.; Ognibene, Marzia; Trielli, Francesca; Politi, Huguette; Passalacqua, Mario; Falugi, Carla.

In: Neuroscience Letters, Vol. 329, No. 1, 23.08.2002, p. 65-68.

Research output: Contribution to journalArticle

Delmonte Corrado, Maria U. ; Ognibene, Marzia ; Trielli, Francesca ; Politi, Huguette ; Passalacqua, Mario ; Falugi, Carla. / Detection of molecules related to the GABAergic system in a single-cell eukaryote, Paramecium primaurelia. In: Neuroscience Letters. 2002 ; Vol. 329, No. 1. pp. 65-68.
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