Abstract
Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 73-78 |
| Number of pages | 6 |
| Journal | APMIS |
| Volume | 109 |
| Issue number | 1 |
| Publication status | Published - 2001 |
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Keywords
- HUVEC
- mRNA
- TCC
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Microbiology (medical)
- Immunology
Cite this
Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro. / Langeggen, H.; Berg, K. E.; Macor, P.; Fischetti, F.; Tedesco, F.; Hetland, G.; Berg, K.; Johnson, E.
In: APMIS, Vol. 109, No. 1, 2001, p. 73-78.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro
AU - Langeggen, H.
AU - Berg, K. E.
AU - Macor, P.
AU - Fischetti, F.
AU - Tedesco, F.
AU - Hetland, G.
AU - Berg, K.
AU - Johnson, E.
PY - 2001
Y1 - 2001
N2 - Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.
AB - Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.
KW - HUVEC
KW - mRNA
KW - TCC
UR - http://www.scopus.com/inward/record.url?scp=0035076705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035076705&partnerID=8YFLogxK
M3 - Article
C2 - 11297196
AN - SCOPUS:0035076705
VL - 109
SP - 73
EP - 78
JO - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology
JF - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology
SN - 0365-5555
IS - 1
ER -