Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro

H. Langeggen, K. E. Berg, P. Macor, F. Fischetti, F. Tedesco, G. Hetland, K. Berg, E. Johnson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Human umbilical vein endothelial cells (HUVEC) have previously been shown to synthesize the functional terminal pathway of complement based on the detection by radioimmunoassay of the terminal complement complex (TCC) on coincubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. However, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined whether mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) and Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalAPMIS
Volume109
Issue number1
Publication statusPublished - 2001

Fingerprint

Complement C5
Human Umbilical Vein Endothelial Cells
Messenger RNA
Complement Membrane Attack Complex
Northern Blotting
Sepharose
Reverse Transcription
Radioimmunoassay
Polymerase Chain Reaction
In Vitro Techniques

Keywords

  • HUVEC
  • mRNA
  • TCC

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)
  • Immunology

Cite this

Langeggen, H., Berg, K. E., Macor, P., Fischetti, F., Tedesco, F., Hetland, G., ... Johnson, E. (2001). Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro. APMIS, 109(1), 73-78.

Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro. / Langeggen, H.; Berg, K. E.; Macor, P.; Fischetti, F.; Tedesco, F.; Hetland, G.; Berg, K.; Johnson, E.

In: APMIS, Vol. 109, No. 1, 2001, p. 73-78.

Research output: Contribution to journalArticle

Langeggen, H, Berg, KE, Macor, P, Fischetti, F, Tedesco, F, Hetland, G, Berg, K & Johnson, E 2001, 'Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro', APMIS, vol. 109, no. 1, pp. 73-78.
Langeggen, H. ; Berg, K. E. ; Macor, P. ; Fischetti, F. ; Tedesco, F. ; Hetland, G. ; Berg, K. ; Johnson, E. / Detection of mRNA for the terminal complement components C5, C6, C8 and C9 in human umbilical vein endothelial cells in vitro. In: APMIS. 2001 ; Vol. 109, No. 1. pp. 73-78.
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