Abstract
Background: Epidemiologic evidence points to a connection between viral infections by the human papillomavirus (HPV) and a subgroup of squamous cell carcinomas of the oropharynx. Still controversial is the association of HPV infection with oesophageal neoplasia. Objectives: To investigate the presence of mucosal as well as cutaneous HPVs in squamous cell carcinoma and adenocarcinoma of the oesophagus. Study design: HPV DNA has been searched by PCR and characterized by nucleotide sequence analysis in paraffin-embedded biopsies from Italian patients with oesophageal squamous cell carcinoma (n = 36), sarcomatoid cell carcinoma (n = 1), adenocarcinoma (n = 20) and oesophagitis lesions (n = 27). Results: A broad spectrum of HPVs, primarily cutaneous types was demonstrated in 27.8% (10/36) of squamous cell carcinomas with a significantly higher frequency in well (G1) and moderately (G2) differentiated grades (47.3%, 9/19) compared to poorly (G3) differentiated (5.9%, 1/17) squamous cell carcinoma (p = 0.008), and in 10% (2/20) of adenocarcinomas and in 29.6% (8/27) of oesophagitis. HPV types detected included mucosal types HPV 6 and 16, cutaneous types HPV 8, 15, 20 and 25; and the putative new HPV types X14, X15, DL473, PPHL1FR and CJ198. Conclusions: There is no evidence of any association between mucosal HPVs and oesophageal neoplasia. The cutaneous HPVs are detected at low frequency in adenocarcinoma and poorly differentiated squamous cell carcinoma, while they are frequently detected in oesophagitis and in well and moderately differentiated squamous cell carcinoma suggesting their tropism for keratinized tissue, although a significant association with such neoplasias cannot be drawn.
| Original language | English |
|---|---|
| Pages (from-to) | 28-33 |
| Number of pages | 6 |
| Journal | Journal of Clinical Virology |
| Volume | 45 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 2009 |
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Keywords
- Cutaneous human papillomaviruses
- Italy
- Mucosal human papillomaviruses
- Oesophageal adenocarcinoma
- Oesophageal squamous cell carcinoma
- Oesophagitis
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Detection of mucosal and cutaneous human papillomaviruses in oesophagitis, squamous cell carcinoma and adenocarcinoma of the oesophagus. / Tornesello, Maria Lina; Monaco, Roberto; Nappi, Oscar; Buonaguro, Luigi; Buonaguro, Franco Maria.
In: Journal of Clinical Virology, Vol. 45, No. 1, 05.2009, p. 28-33.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of mucosal and cutaneous human papillomaviruses in oesophagitis, squamous cell carcinoma and adenocarcinoma of the oesophagus
AU - Tornesello, Maria Lina
AU - Monaco, Roberto
AU - Nappi, Oscar
AU - Buonaguro, Luigi
AU - Buonaguro, Franco Maria
PY - 2009/5
Y1 - 2009/5
N2 - Background: Epidemiologic evidence points to a connection between viral infections by the human papillomavirus (HPV) and a subgroup of squamous cell carcinomas of the oropharynx. Still controversial is the association of HPV infection with oesophageal neoplasia. Objectives: To investigate the presence of mucosal as well as cutaneous HPVs in squamous cell carcinoma and adenocarcinoma of the oesophagus. Study design: HPV DNA has been searched by PCR and characterized by nucleotide sequence analysis in paraffin-embedded biopsies from Italian patients with oesophageal squamous cell carcinoma (n = 36), sarcomatoid cell carcinoma (n = 1), adenocarcinoma (n = 20) and oesophagitis lesions (n = 27). Results: A broad spectrum of HPVs, primarily cutaneous types was demonstrated in 27.8% (10/36) of squamous cell carcinomas with a significantly higher frequency in well (G1) and moderately (G2) differentiated grades (47.3%, 9/19) compared to poorly (G3) differentiated (5.9%, 1/17) squamous cell carcinoma (p = 0.008), and in 10% (2/20) of adenocarcinomas and in 29.6% (8/27) of oesophagitis. HPV types detected included mucosal types HPV 6 and 16, cutaneous types HPV 8, 15, 20 and 25; and the putative new HPV types X14, X15, DL473, PPHL1FR and CJ198. Conclusions: There is no evidence of any association between mucosal HPVs and oesophageal neoplasia. The cutaneous HPVs are detected at low frequency in adenocarcinoma and poorly differentiated squamous cell carcinoma, while they are frequently detected in oesophagitis and in well and moderately differentiated squamous cell carcinoma suggesting their tropism for keratinized tissue, although a significant association with such neoplasias cannot be drawn.
AB - Background: Epidemiologic evidence points to a connection between viral infections by the human papillomavirus (HPV) and a subgroup of squamous cell carcinomas of the oropharynx. Still controversial is the association of HPV infection with oesophageal neoplasia. Objectives: To investigate the presence of mucosal as well as cutaneous HPVs in squamous cell carcinoma and adenocarcinoma of the oesophagus. Study design: HPV DNA has been searched by PCR and characterized by nucleotide sequence analysis in paraffin-embedded biopsies from Italian patients with oesophageal squamous cell carcinoma (n = 36), sarcomatoid cell carcinoma (n = 1), adenocarcinoma (n = 20) and oesophagitis lesions (n = 27). Results: A broad spectrum of HPVs, primarily cutaneous types was demonstrated in 27.8% (10/36) of squamous cell carcinomas with a significantly higher frequency in well (G1) and moderately (G2) differentiated grades (47.3%, 9/19) compared to poorly (G3) differentiated (5.9%, 1/17) squamous cell carcinoma (p = 0.008), and in 10% (2/20) of adenocarcinomas and in 29.6% (8/27) of oesophagitis. HPV types detected included mucosal types HPV 6 and 16, cutaneous types HPV 8, 15, 20 and 25; and the putative new HPV types X14, X15, DL473, PPHL1FR and CJ198. Conclusions: There is no evidence of any association between mucosal HPVs and oesophageal neoplasia. The cutaneous HPVs are detected at low frequency in adenocarcinoma and poorly differentiated squamous cell carcinoma, while they are frequently detected in oesophagitis and in well and moderately differentiated squamous cell carcinoma suggesting their tropism for keratinized tissue, although a significant association with such neoplasias cannot be drawn.
KW - Cutaneous human papillomaviruses
KW - Italy
KW - Mucosal human papillomaviruses
KW - Oesophageal adenocarcinoma
KW - Oesophageal squamous cell carcinoma
KW - Oesophagitis
UR - http://www.scopus.com/inward/record.url?scp=67349097777&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349097777&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2009.02.004
DO - 10.1016/j.jcv.2009.02.004
M3 - Article
C2 - 19307150
AN - SCOPUS:67349097777
VL - 45
SP - 28
EP - 33
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 1
ER -