Detection of mucus glycoconjugates in human conjuctiva by using the lectin-colloidal gold technique in TEM. II. A quantitative study in dry-eye patients

P. Versura, M. C. Maltarello, M. Cellini, R. Caramazza, R. Laschi

Research output: Contribution to journalArticlepeer-review

Abstract

The mucus glycoconjugates produced by conjuctival goblet cells in dry-eye patients were studied by a specific cytochemical reaction in Transmission Electron Microscopy (TEM). Four lectins, proteins of vegetal origin which specifically bind glycosidic residues, (WGA, PNA, SBA and ConA) were used conjugated with colloidal gold as ultrastructural marker. We performed a quantitative analysis by counting the colloidal gold particles present on mucus granules. The results were compared with normal conditions. We found a decrease in sialic acid, N-acetyl-glucosamine, N-acetyl-galactosamine and galactose-N-acetyl-galactosamine and an increase in mannose. The different content of glycoconjugates in goblet cells may reflect in the change of physical and functional properties of mucus. We think these data may be useful in the search for a therapeutic mucomimetic drug.

Original languageEnglish
Pages (from-to)451-455
Number of pages5
JournalActa Ophthalmologica
Volume64
Issue number4
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Ophthalmology

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