Ricerca del DNA del Mycobacterium tuberculosis mediante nested Polymerase Chain Reaction in linfonodi con sarcoidosi fissati in formalina e inclusi in paraffina

Translated title of the contribution: Detection of Mycobacterium tuberculosis DNA in formalin-fixed, paraffin-embedded lymphnodes with sarcoidosis by nested Polymerase Chain Reaction

M. Cannone, L. Vago, G. Porini, S. Bonetto, C. Cassi, M. Bramerio, G. Rizzato, M. C P Barberis

Research output: Contribution to journalArticlepeer-review

Abstract

An involvement of mycobacteria in the pathogenesis of sarcoidosis has often been hypothesized, but not confirmed reproducibly. In this study we applied a nested Polymerase Chain Reaction (PCR) to the insertion sequence IS6110 for detection of Mycobacterium tuberculosis (MT) DNA in formalin-fixed and paraffin-embedded tissues from patients with sarcoidosis, with tuberculosis and atypical mycobacteriosis confirmed by culture, and from negative control samples. MT- DNA could be detected in 2/30 samples of sarcoidosis, in 10/10 tuberculoses, in 0/5 atypical mycobacterioses and in 0/ 10 negative controls. Nested PCR confirmed its high sensitivity and specificity in detecting MT-DNA on archival histopathological specimens. From our results we conclude that in the granulomatous lesions of sarcoidosis MT-DNA is only sporadically demonstrable and probably it doesn't play a role in the pathogenesis of this disease.

Translated title of the contributionDetection of Mycobacterium tuberculosis DNA in formalin-fixed, paraffin-embedded lymphnodes with sarcoidosis by nested Polymerase Chain Reaction
Original languageItalian
Pages (from-to)512-516
Number of pages5
JournalPathologica
Volume89
Issue number5
Publication statusPublished - Oct 1997

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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