Abstract
The aim of the present study was to evaluate the nitric oxide (NO) concentrations present in end-expired gas (FENO) of different animal species under basal and stimulated conditions using a clinical chemiluminescence analyser, which has been developed for measurement of single exhalations in humans. Anaesthetised, tracheotomised and artificially ventilated guinea pigs, rats and rabbits were prepared for recording systemic blood pressure and FENO. Stable levels of FENO were detected in expired air over a 1-h observation period in the three animal species tested. Rabbits exhibited the highest concentrations and output (FENO 12.9 ± 1.0 ppb, VNO 9.0 ± 0.7 nl min-1), followed by guinea pigs (FENO 6.2 ± 0.70 ppb, VNO 1.7 ± 0.19 nl min-1) and rats (FENO 0.9 ± 0.01 ppb, VNO 0.25 ± 0.00 nl min-1). L-Arginine (1 g kg-1 i.v.) evoked significant increments in VNO in guinea pigs and rabbits but was ineffective in rats. However, L-arginine showed a direct effect on blood pressure in all the animal species tested, causing a rapid fall in the mean arterial blood pressure (MABP; 38, 48 and 50% decrease in rabbits, guinea pigs and rats, respectively; P <0.05). An inhibitor of endogenous NO synthesis, N(G)-nitro-L-arginine methyl ester (L-NAME, 20 mg kg-1 i.v.), decreased both basal and L-arginine-induced VNO in guinea pigs and rabbits, but was ineffective in rats. L-NAME increased MABP in all the animal species tested (58% in guinea pigs, 43% in rats and 18% in rabbits; P <0.05). The results indicate that it is possible to detect NO in the exhaled air of different animal species using a clinical chemiluminescence analyser and that different species exhibit striking differences in the levels of basal and stimulated NO output.
| Original language | English |
|---|---|
| Pages (from-to) | 221-224 |
| Number of pages | 4 |
| Journal | Pharmacological Research |
| Volume | 39 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 1999 |
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Keywords
- Chemiluminescence analyser
- Exhaled air
- Nitric oxide (NO)
ASJC Scopus subject areas
- Pharmacology
Cite this
Detection of nitric oxide in exhaled air of different animal species using a clinical chemiluminescence analyser. / Bernareggi, M.; Rossoni, G.; Clini, E.; Pasini, E.; Bachetti, T.; Cremona, G.; Ambrosino, N.; Berti, F.
In: Pharmacological Research, Vol. 39, No. 3, 03.1999, p. 221-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of nitric oxide in exhaled air of different animal species using a clinical chemiluminescence analyser
AU - Bernareggi, M.
AU - Rossoni, G.
AU - Clini, E.
AU - Pasini, E.
AU - Bachetti, T.
AU - Cremona, G.
AU - Ambrosino, N.
AU - Berti, F.
PY - 1999/3
Y1 - 1999/3
N2 - The aim of the present study was to evaluate the nitric oxide (NO) concentrations present in end-expired gas (FENO) of different animal species under basal and stimulated conditions using a clinical chemiluminescence analyser, which has been developed for measurement of single exhalations in humans. Anaesthetised, tracheotomised and artificially ventilated guinea pigs, rats and rabbits were prepared for recording systemic blood pressure and FENO. Stable levels of FENO were detected in expired air over a 1-h observation period in the three animal species tested. Rabbits exhibited the highest concentrations and output (FENO 12.9 ± 1.0 ppb, VNO 9.0 ± 0.7 nl min-1), followed by guinea pigs (FENO 6.2 ± 0.70 ppb, VNO 1.7 ± 0.19 nl min-1) and rats (FENO 0.9 ± 0.01 ppb, VNO 0.25 ± 0.00 nl min-1). L-Arginine (1 g kg-1 i.v.) evoked significant increments in VNO in guinea pigs and rabbits but was ineffective in rats. However, L-arginine showed a direct effect on blood pressure in all the animal species tested, causing a rapid fall in the mean arterial blood pressure (MABP; 38, 48 and 50% decrease in rabbits, guinea pigs and rats, respectively; P <0.05). An inhibitor of endogenous NO synthesis, N(G)-nitro-L-arginine methyl ester (L-NAME, 20 mg kg-1 i.v.), decreased both basal and L-arginine-induced VNO in guinea pigs and rabbits, but was ineffective in rats. L-NAME increased MABP in all the animal species tested (58% in guinea pigs, 43% in rats and 18% in rabbits; P <0.05). The results indicate that it is possible to detect NO in the exhaled air of different animal species using a clinical chemiluminescence analyser and that different species exhibit striking differences in the levels of basal and stimulated NO output.
AB - The aim of the present study was to evaluate the nitric oxide (NO) concentrations present in end-expired gas (FENO) of different animal species under basal and stimulated conditions using a clinical chemiluminescence analyser, which has been developed for measurement of single exhalations in humans. Anaesthetised, tracheotomised and artificially ventilated guinea pigs, rats and rabbits were prepared for recording systemic blood pressure and FENO. Stable levels of FENO were detected in expired air over a 1-h observation period in the three animal species tested. Rabbits exhibited the highest concentrations and output (FENO 12.9 ± 1.0 ppb, VNO 9.0 ± 0.7 nl min-1), followed by guinea pigs (FENO 6.2 ± 0.70 ppb, VNO 1.7 ± 0.19 nl min-1) and rats (FENO 0.9 ± 0.01 ppb, VNO 0.25 ± 0.00 nl min-1). L-Arginine (1 g kg-1 i.v.) evoked significant increments in VNO in guinea pigs and rabbits but was ineffective in rats. However, L-arginine showed a direct effect on blood pressure in all the animal species tested, causing a rapid fall in the mean arterial blood pressure (MABP; 38, 48 and 50% decrease in rabbits, guinea pigs and rats, respectively; P <0.05). An inhibitor of endogenous NO synthesis, N(G)-nitro-L-arginine methyl ester (L-NAME, 20 mg kg-1 i.v.), decreased both basal and L-arginine-induced VNO in guinea pigs and rabbits, but was ineffective in rats. L-NAME increased MABP in all the animal species tested (58% in guinea pigs, 43% in rats and 18% in rabbits; P <0.05). The results indicate that it is possible to detect NO in the exhaled air of different animal species using a clinical chemiluminescence analyser and that different species exhibit striking differences in the levels of basal and stimulated NO output.
KW - Chemiluminescence analyser
KW - Exhaled air
KW - Nitric oxide (NO)
UR - http://www.scopus.com/inward/record.url?scp=0033105416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033105416&partnerID=8YFLogxK
U2 - 10.1006/phrs.1998.0428
DO - 10.1006/phrs.1998.0428
M3 - Article
C2 - 10094848
AN - SCOPUS:0033105416
VL - 39
SP - 221
EP - 224
JO - Pharmacological Research
JF - Pharmacological Research
SN - 1043-6618
IS - 3
ER -