Detection of normal and chimeric nucleophosmin in human cells

Jacqueline L. Cordell, Karen A F Pulford, Barbara Bigerna, Giovanna Roncador, Alison Banham, Emanuela Colombo, Pier Giuseppe Pelicci, David Y. Mason, Brunangelo Falini

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

In anaplastic large-cell lymphoma (ALCL), the (2;5) chromosomal translocation creates a fusion gene encoding the 80-kD NPM-ALK hybrid protein. This report describes three new monoclonal antibodies, two of which recognize, by Western blotting, the N-terminal portion of NPM present in the NPM-ALK fusion protein and also in two other NPM fusion proteins (NPM-RARα and NPM-MLF1). The third antibody recognizes the C-terminal portion (deleted in NPMALK) and reacts only with wild-type NPM. The three antibodies immunostain wild-type NPM (in paraffin-embedded normal tissue samples) in cell nuclei and in the cytoplasm of mitotic cells. Cerebral neurones, exceptionally, show diffuse cytoplasmic labeling. In contrast to normal tissues, the two antibodies against the N-terminal portion of NPM labeled the cytoplasm of neoplastic cells, in four ALK-positive ALCL, reflecting their reactivity with NPM-ALK fusion protein, whereas the antibody to the C- terminal NPM epitope labeled only cell nuclei. Immunocytochemical labeling with these antibodies can therefore confirm that an ALK-positive lymphoma expresses NPM-ALK (rather than a variant ALK-fusion protein) and may also provide evidence for chromosomal anomalies involving the NPM gene other than the classical (2;5) translocation.

Original languageEnglish
Pages (from-to)632-642
Number of pages11
JournalBlood
Volume93
Issue number2
Publication statusPublished - Jan 15 1999

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Cells
Antibodies
Anaplastic Large-Cell Lymphoma
Fusion reactions
Cell Nucleus
Labeling
Cytoplasm
Tissue
Genetic Translocation
Proteins
Gene encoding
Gene Fusion
Paraffin
Neurons
Epitopes
Lymphoma
Genes
Western Blotting
Monoclonal Antibodies
nucleophosmin

ASJC Scopus subject areas

  • Hematology

Cite this

Cordell, J. L., Pulford, K. A. F., Bigerna, B., Roncador, G., Banham, A., Colombo, E., ... Falini, B. (1999). Detection of normal and chimeric nucleophosmin in human cells. Blood, 93(2), 632-642.

Detection of normal and chimeric nucleophosmin in human cells. / Cordell, Jacqueline L.; Pulford, Karen A F; Bigerna, Barbara; Roncador, Giovanna; Banham, Alison; Colombo, Emanuela; Pelicci, Pier Giuseppe; Mason, David Y.; Falini, Brunangelo.

In: Blood, Vol. 93, No. 2, 15.01.1999, p. 632-642.

Research output: Contribution to journalArticle

Cordell, JL, Pulford, KAF, Bigerna, B, Roncador, G, Banham, A, Colombo, E, Pelicci, PG, Mason, DY & Falini, B 1999, 'Detection of normal and chimeric nucleophosmin in human cells', Blood, vol. 93, no. 2, pp. 632-642.
Cordell JL, Pulford KAF, Bigerna B, Roncador G, Banham A, Colombo E et al. Detection of normal and chimeric nucleophosmin in human cells. Blood. 1999 Jan 15;93(2):632-642.
Cordell, Jacqueline L. ; Pulford, Karen A F ; Bigerna, Barbara ; Roncador, Giovanna ; Banham, Alison ; Colombo, Emanuela ; Pelicci, Pier Giuseppe ; Mason, David Y. ; Falini, Brunangelo. / Detection of normal and chimeric nucleophosmin in human cells. In: Blood. 1999 ; Vol. 93, No. 2. pp. 632-642.
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