Detection of platelet-associated antibodies by flow cytometry in hematological autoimmune disorders

P. L. Tazzari, F. Ricci, N. Vianelli, C. Tassi, D. Belletti, I. Pierri, L. Gugliotta, M. Gobbi, R. Conte

Research output: Contribution to journalArticle

Abstract

We describe our experience in the evaluation of platelet-associated immunoglobulins (PAIg) by flow cytometry in comparison to solid-phase assay in patients affected by idiopathic thrombocytopenic purpura and by Evans syndrome. Results show that the analysis of PAIg by flow cytometry is easy and reliable and correlates well with data obtained by the solid-phase technique. In addition, flow cytometry allows the evaluation of samples containing small numbers of platelets (3); the analysis is objective, not influenced by personal experience. Moreover, flow cytometry appears simple enough to be performed in a routine laboratory, and data might be retrieved to perform batch analysis. Our results appear to indicate that PAIg flow cytometry might be a sensitive tool for the evaluation of patients with autoimmune thrombocytopenia.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalAnnals of Hematology
Volume70
Issue number5
DOIs
Publication statusPublished - May 1995

Keywords

  • Autoimmunity
  • Flow cytometry
  • Idiopathic thrombocytopenic purpura (ITP)
  • Platelet-associated immunoglobulins (PAIg)
  • Platelets

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Detection of platelet-associated antibodies by flow cytometry in hematological autoimmune disorders'. Together they form a unique fingerprint.

  • Cite this

    Tazzari, P. L., Ricci, F., Vianelli, N., Tassi, C., Belletti, D., Pierri, I., Gugliotta, L., Gobbi, M., & Conte, R. (1995). Detection of platelet-associated antibodies by flow cytometry in hematological autoimmune disorders. Annals of Hematology, 70(5), 267-272. https://doi.org/10.1007/BF01784046