Detection of Pneumocystis carinii in respiratory specimens by PCR- solution hybridization enzyme-linked immunoassay

Elena Ortona, Paola Margutti, Enrica Tamburrini, Paola Mencarini, Elena Visconti, Maria Zolfo, Alessandra Siracusano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

By using a recently developed PCR-solution hybridization enzyme-linked assay (PCR-SHELA), we investigated Pneumocystis carinii in bronchoalveolar lavage fluid samples and induced sputa of patients with pneumocystosis. In detecting P. carinii, PCR-SHELA proved more sensitive than immunofluorescence staining or a single PCR and significantly more diagnostically specific than a nested PCR. Our data suggest that PCR-SHELA could be used to detect P. carinii organisms in respiratory samples, particularly in patients with uncertain diagnoses.

Original languageEnglish
Pages (from-to)1589-1591
Number of pages3
JournalJournal of Clinical Microbiology
Volume35
Issue number6
Publication statusPublished - Jun 1997

Fingerprint

Pneumocystis carinii
Immunoenzyme Techniques
Enzyme Assays
Polymerase Chain Reaction
Pneumocystis Pneumonia
Bronchoalveolar Lavage Fluid
Sputum
Fluorescent Antibody Technique
Staining and Labeling

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Ortona, E., Margutti, P., Tamburrini, E., Mencarini, P., Visconti, E., Zolfo, M., & Siracusano, A. (1997). Detection of Pneumocystis carinii in respiratory specimens by PCR- solution hybridization enzyme-linked immunoassay. Journal of Clinical Microbiology, 35(6), 1589-1591.

Detection of Pneumocystis carinii in respiratory specimens by PCR- solution hybridization enzyme-linked immunoassay. / Ortona, Elena; Margutti, Paola; Tamburrini, Enrica; Mencarini, Paola; Visconti, Elena; Zolfo, Maria; Siracusano, Alessandra.

In: Journal of Clinical Microbiology, Vol. 35, No. 6, 06.1997, p. 1589-1591.

Research output: Contribution to journalArticle

Ortona, E, Margutti, P, Tamburrini, E, Mencarini, P, Visconti, E, Zolfo, M & Siracusano, A 1997, 'Detection of Pneumocystis carinii in respiratory specimens by PCR- solution hybridization enzyme-linked immunoassay', Journal of Clinical Microbiology, vol. 35, no. 6, pp. 1589-1591.
Ortona, Elena ; Margutti, Paola ; Tamburrini, Enrica ; Mencarini, Paola ; Visconti, Elena ; Zolfo, Maria ; Siracusano, Alessandra. / Detection of Pneumocystis carinii in respiratory specimens by PCR- solution hybridization enzyme-linked immunoassay. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 6. pp. 1589-1591.
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