Detection of Pneumocystis carinii in respiratory specimens by PCR- solution hybridization enzyme-linked immunoassay

Elena Ortona, Paola Margutti, Enrica Tamburrini, Paola Mencarini, Elena Visconti, Maria Zolfo, Alessandra Siracusano

Research output: Contribution to journalArticle

Abstract

By using a recently developed PCR-solution hybridization enzyme-linked assay (PCR-SHELA), we investigated Pneumocystis carinii in bronchoalveolar lavage fluid samples and induced sputa of patients with pneumocystosis. In detecting P. carinii, PCR-SHELA proved more sensitive than immunofluorescence staining or a single PCR and significantly more diagnostically specific than a nested PCR. Our data suggest that PCR-SHELA could be used to detect P. carinii organisms in respiratory samples, particularly in patients with uncertain diagnoses.

Original languageEnglish
Pages (from-to)1589-1591
Number of pages3
JournalJournal of Clinical Microbiology
Volume35
Issue number6
Publication statusPublished - Jun 1997

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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