Detection of post-transcriptional RNA editing events

Ernesto Picardi, Anna Maria D'Erchia, Angela Gallo, Graziano Pesole

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The advent of deep sequencing technologies has greatly improved the study of complex eukaryotic genomes and transcriptomes, providing the unique opportunity to investigate posttranscriptional molecular mechanisms as alternative splicing and RNA editing at single base-pair resolution. RNA editing by adenosine deamination (A-to-I) is widespread in humans and can lead to a variety of biological effects depending on the RNA type or the RNA region involved in the editing modification. Hereafter, we describe an easy and reproducible computational protocol for the identification of candidate RNA editing sites in human using deep transcriptome (RNA-Seq) and genome (DNA-Seq) sequencing data.

Original languageEnglish
Title of host publicationRNA Bioinformatics
PublisherSpringer New York
Pages189-205
Number of pages17
ISBN (Print)9781493922918, 9781493922901
DOIs
Publication statusPublished - Jan 10 2015

Keywords

  • A-to-I editing
  • Bioinformatics
  • Deep sequencing
  • DNA-seq
  • Genomics
  • RNA editing
  • RNA-seq

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Picardi, E., D'Erchia, A. M., Gallo, A., & Pesole, G. (2015). Detection of post-transcriptional RNA editing events. In RNA Bioinformatics (pp. 189-205). Springer New York. https://doi.org/10.1007/978-1-4939-2291-8_12