Resistance of H. pylori to clarithromycin is a major cause of the failure of eradication therapy. The mechanism is mediated by point mutations in 23S rRNA gene. To evaluate the frequency of macrolide resistance in dyspetic patients and find a method for detecting the mutations in gastric biopsies. The tecnique involves amplifying a 268-bp sequence of 23S rRNA and identifying mutations by a double gradient-denaturing gradient gel electrophoresis (DG-DGGE). Thirty-eight consecutive dyspeptic patients entered in the study. Biopsies specimens were collected during endoscopy and tested for microbiological methods (histological examinations and cultures) and molecular assay. Twenty-six patients (68,4%) were found positive for H. pylori on conventional methods and/or 23S rRNA PCR DG-DGGE analysis revealed mutations (clarithromycin resistance) in twenty-three subjects (88,5%). This molecular assay proved to be rapid and reliable test; its use in clinical practice should be evaluated.
|Translated title of the contribution||Detection of puntiform mutations associated with clarithromycin resistance in dyspeptic patients with Helicobacter pylori infection|
|Number of pages||3|
|Journal||Argomenti di Gastroenterologia Clinica|
|Publication status||Published - Mar 2002|
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