Detection of rat Pneumocystis carinii proteinases and elastase and antipneumocystis activity of proteinase inhibitors in vitro

Chiara Atzori, A. Mainini, F. Agostoni, E. Angeli, M. Bartlett, A. Bruno, M. Scaglia, A. Cargnel

Research output: Contribution to journalArticle

Abstract

Proteinases play an important role in survival of microorganisms and in pathogenicity of diseases. By using a modified SDS-gelatin-polyacrylamide gel system, proteinases of rat-P.carinii were detected as bands of proteolytic digestion after electrophoresis. P.carinii organisms obtained from dexamethazone immuno-suppressed transtracheally infected rats were cultured in spinner flask suspension cultures to minimize host cell contamination. At pH 8.3, seven Pc-specific proteolytic bands were detected in three clusters of different molecular weights clearly different from host cell patterns. By using a range of pH, various preparations of organisms and both infected and uninfected culture media, proteolytic activities have been partially characterized. Elastase secretion has been assessed based on elastin digestion model. Proteinase inhibitors have been tested for their ability to inhibit P.carinii growth in HEL299 short-term monolayer cultures. Results indicate that proteolytic activities are involved in the proliferation of microorganisms since leupeptin exerted in vitro antipneumocystis activity while aprotinin enhanced P.carinii growth.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalParasite (Paris, France)
Volume6
Issue number1
Publication statusPublished - Mar 1999

Keywords

  • Elastase
  • P. carinii proteinases
  • Proteinase inhibitors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology

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    Atzori, C., Mainini, A., Agostoni, F., Angeli, E., Bartlett, M., Bruno, A., Scaglia, M., & Cargnel, A. (1999). Detection of rat Pneumocystis carinii proteinases and elastase and antipneumocystis activity of proteinase inhibitors in vitro. Parasite (Paris, France), 6(1), 9-16.