Detection of replicative intermediates of viral RNA in peripheral blood mononuclear cells from chronic hepatitis C virus carriers.

M. Artini, G. Natoli, M. L. Avantaggiati, C. Balsano, P. Chirillo, A. Costanzo, M. S. Bonavita, M. Levrero

Research output: Contribution to journalArticle

Abstract

Clinical and experimental evidence suggests the possible existence of one or more extrahepatic sites of HCV infection. In order to demonstrate the "in vivo" infection of lymphoid cells by HCV, we applied a nested PCR to total cytoplasmic RNA extracted from fresh or cultured peripheral blood mononuclear cells (PBMCs) of HCV chronically infected patients, using primers derived from the highly conserved 5' untranslated region of the HCV genome. The presence of virions in PBMCs occurs frequently, if not always, and is often accompanied by active viral replication. Moreover, the appearance of replicative intermediates after stimulation of cellular growth with mitogens suggests that latent genomes could undergo replication upon cellular activation and/or proliferation.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalArchives of virology. Supplementum
Volume8
Publication statusPublished - 1993

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Artini, M., Natoli, G., Avantaggiati, M. L., Balsano, C., Chirillo, P., Costanzo, A., Bonavita, M. S., & Levrero, M. (1993). Detection of replicative intermediates of viral RNA in peripheral blood mononuclear cells from chronic hepatitis C virus carriers. Archives of virology. Supplementum, 8, 23-29.