Abstract
We applied double gradient-denaturing gradient gel electrophoresis (DG- DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 2550-2554 |
| Number of pages | 5 |
| Journal | Antimicrobial Agents and Chemotherapy |
| Volume | 43 |
| Issue number | 10 |
| Publication status | Published - 1999 |
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ASJC Scopus subject areas
- Pharmacology (medical)
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Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. / Scarpellini, Paolo; Braglia, Sergio; Carrera, Paola; Cedri, Maura; Cichero, Paola; Colombo, Alessia; Crucianelli, Rosella; Gori, Andrea; Ferrari, Maurizio; Lazzarin, Adriano.
In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 10, 1999, p. 2550-2554.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis
AU - Scarpellini, Paolo
AU - Braglia, Sergio
AU - Carrera, Paola
AU - Cedri, Maura
AU - Cichero, Paola
AU - Colombo, Alessia
AU - Crucianelli, Rosella
AU - Gori, Andrea
AU - Ferrari, Maurizio
AU - Lazzarin, Adriano
PY - 1999
Y1 - 1999
N2 - We applied double gradient-denaturing gradient gel electrophoresis (DG- DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.
AB - We applied double gradient-denaturing gradient gel electrophoresis (DG- DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.
UR - http://www.scopus.com/inward/record.url?scp=0032870373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032870373&partnerID=8YFLogxK
M3 - Article
C2 - 10508043
AN - SCOPUS:0032870373
VL - 43
SP - 2550
EP - 2554
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 10
ER -