Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis

Paolo Scarpellini, Sergio Braglia, Paola Carrera, Maura Cedri, Paola Cichero, Alessia Colombo, Rosella Crucianelli, Andrea Gori, Maurizio Ferrari, Adriano Lazzarin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We applied double gradient-denaturing gradient gel electrophoresis (DG- DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

Original languageEnglish
Pages (from-to)2550-2554
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume43
Issue number10
Publication statusPublished - 1999

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Denaturing Gradient Gel Electrophoresis
Rifampin
Mycobacterium tuberculosis
DNA Sequence Analysis
Polymerase Chain Reaction
Mutation

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Scarpellini, P., Braglia, S., Carrera, P., Cedri, M., Cichero, P., Colombo, A., ... Lazzarin, A. (1999). Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. Antimicrobial Agents and Chemotherapy, 43(10), 2550-2554.

Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. / Scarpellini, Paolo; Braglia, Sergio; Carrera, Paola; Cedri, Maura; Cichero, Paola; Colombo, Alessia; Crucianelli, Rosella; Gori, Andrea; Ferrari, Maurizio; Lazzarin, Adriano.

In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 10, 1999, p. 2550-2554.

Research output: Contribution to journalArticle

Scarpellini, P, Braglia, S, Carrera, P, Cedri, M, Cichero, P, Colombo, A, Crucianelli, R, Gori, A, Ferrari, M & Lazzarin, A 1999, 'Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis', Antimicrobial Agents and Chemotherapy, vol. 43, no. 10, pp. 2550-2554.
Scarpellini, Paolo ; Braglia, Sergio ; Carrera, Paola ; Cedri, Maura ; Cichero, Paola ; Colombo, Alessia ; Crucianelli, Rosella ; Gori, Andrea ; Ferrari, Maurizio ; Lazzarin, Adriano. / Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. In: Antimicrobial Agents and Chemotherapy. 1999 ; Vol. 43, No. 10. pp. 2550-2554.
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