Detection of SHV-5 extended-spectrum beta-lactamase in Klebsiella pneumoniae strains isolated in Italy

A. Marchese, G. Arlet, G. C. Schito, P. H. Lagrange, A. Philippon

Research output: Contribution to journalArticlepeer-review

Abstract

Thirty-five Klebsiella pneumoniae strains isolated during 1993-1994 in intensive cave units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination.

Original languageEnglish
Pages (from-to)245-248
Number of pages4
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume15
Issue number3
DOIs
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)

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