Detection of SHV-5 extended-spectrum beta-lactamase in Klebsiella pneumoniae strains isolated in Italy

A. Marchese; G. Arlet; G. C. Schito; P. H. Lagrange; A. Philippon

doi:10.1007/BF01591363

Detection of SHV-5 extended-spectrum beta-lactamase in Klebsiella pneumoniae strains isolated in Italy

A. Marchese, G. Arlet, G. C. Schito, P. H. Lagrange, A. Philippon

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

Thirty-five Klebsiella pneumoniae strains isolated during 1993-1994 in intensive cave units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination.

Original language	English
Pages (from-to)	245-248
Number of pages	4
Journal	European Journal of Clinical Microbiology and Infectious Diseases
Volume	15
Issue number	3
DOIs	https://doi.org/10.1007/BF01591363
Publication status	Published - 1996

ASJC Scopus subject areas

Immunology
Microbiology (medical)

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title = "Detection of SHV-5 extended-spectrum beta-lactamase in Klebsiella pneumoniae strains isolated in Italy",

abstract = "Thirty-five Klebsiella pneumoniae strains isolated during 1993-1994 in intensive cave units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination.",

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AU - Marchese, A.

AU - Arlet, G.

AU - Schito, G. C.

AU - Lagrange, P. H.

AU - Philippon, A.

PY - 1996

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N2 - Thirty-five Klebsiella pneumoniae strains isolated during 1993-1994 in intensive cave units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination.

AB - Thirty-five Klebsiella pneumoniae strains isolated during 1993-1994 in intensive cave units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination.

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Detection of SHV-5 extended-spectrum beta-lactamase in Klebsiella pneumoniae strains isolated in Italy

Abstract

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