Thirty-five Klebsiella pneumoniae strains isolated during 1993-1994 in intensive cave units of a large Italian hospital were examined for the presence of extended-spectrum β-lactamases. Five strains showed a high level of simultaneous resistance to β-lactam agents, including ceftazidime and aztreonam, conferred by a large (130 kb) self-transferable plasmid (in 4 of 5 strains). Isoelectrofocusing and hybridisation studies suggest that these enzymes can be identified as SHV-5 extended-spectrum β-lactamases. Pulsed-field gel electrophoresis analysis showed three different genomic fingerprinting profiles, while plasmid restriction enzyme digestion revealed three different patterns, demonstrating that the diffusion of SHV-5 β-lactamase is not the result of a single strain or plasmid dissemination.
|Number of pages||4|
|Journal||European Journal of Clinical Microbiology and Infectious Diseases|
|Publication status||Published - 1996|
ASJC Scopus subject areas
- Microbiology (medical)