Detection of soluble ERBB2 in breast cancer cell lysates using a combined label-free/fluorescence platform based on Bloch surface waves

A. Sinibaldi, C. Sampaoli, N. Danz, P. Munzert, L. Sibilio, F. Sonntag, A. Occhicone, E. Falvo, E. Tremante, P. Giacomini, F. Michelotti

Research output: Contribution to journalArticlepeer-review

Abstract

We report on the use of one-dimensional photonic crystals to detect clinically relevant concentrations of ERBB2/neu/Her2 in cell lysates. ERBB2 is a pivotal breast cancer biomarker and targetable oncogenic driver associated with aggressive breast cancer subtypes. To quantitate soluble ERBB2, we developed an optical platform that combines label-free and fluorescence detection modes. Such platform makes use of a sandwich assay in which the one-dimensional photonic crystals sustaining Bloch surface waves are tailored with a monoclonal antibody for highly specific biological recognition (BSW biochip). In a second step, a second antibody to ERBB2 quantitatively detects the bound analyte. The strategy of the present approach takes advantage of the combination of label-free and fluorescence techniques, making bio-recognition more robust and sensitive. In the fluorescence operation mode, the platform can attain the limit of detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates. Such resolution meets the international guidelines and recommendations (15ng/mL) for diagnostic ERBB2 assays that in the future may help to more precisely assign therapies counteracting cancer cell proliferation and metastatic spread.
Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalBiosensors and Bioelectronics
Volume92
DOIs
Publication statusPublished - Jun 15 2017

Keywords

  • Antibodies, Monoclonal/chemistry
  • Biomarkers, Tumor/analysis
  • Biosensing Techniques/instrumentation
  • Breast/pathology
  • Breast Neoplasms/diagnosis
  • Cell Line, Tumor
  • Equipment Design
  • Female
  • Fluorescence
  • Humans
  • Lab-On-A-Chip Devices
  • Limit of Detection
  • Receptor, ErbB-2/analysis
  • 1D photonic crystals
  • Bloch surface waves
  • Breast cancer
  • ERBB2
  • Optical biosensors
  • SK-BR 3

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