Detection of T cell receptor delta gene rearrangement in childhood B and T lineage acute lymphoblastic leukaemia by southern blot and PCR: Technical comparison of two methods of analysis

A. Valetto, D. Di Martino, F. Scuderi, T. Lanza, M. Lanciotti, F. Bottini, G. Dini

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Molecular analysis of antigen receptor genes (Ig and TCR) has been useful for clonal studies in acute lymphoblastic leukaemia (ALL) patients. Rearrangements of these genes can be used to track the persistence of the leukaemic clone during the therapy. The purpose of our study was to analyse the percentage and the pattern of the rearrangements at the TCR D locus in a series of ALL patients, comparing the results obtained by Southern blot and PCR. Genomic DNA was extracted from mononuclear BM cells of 40 paediatric ALL cases, digested with different restriction enzymes and hybridized to TCRDJ1 probe to study the TCR delta locus. Amplification of the rearranged TCR delta genes was performed by PCR to define the gene segments involved. The junctional region was deduced from the sequence to obtain patient-specific primers. Among the 31 B lineage ALL samples, one or two TCR δ alleles proved to be rearranged in 53% of cases. Two different types of rearrangements were chiefly detected: Vδ2Dδ3 and Dδ2Dδ3. In T-ALL patients, the predominant rearrangement involved the Vδ1 and the Jδ1 gene segments.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalClinical and Laboratory Haematology
Volume22
Issue number5
DOIs
Publication statusPublished - 2000

Fingerprint

T-Cell Receptor delta Genes
Gene Rearrangement
Southern Blotting
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Polymerase Chain Reaction
Osteonectin
Genes
Immunoglobulin Genes
Antigen Receptors
Clone Cells
Alleles
Pediatrics
DNA
Enzymes

Keywords

  • Acute lymphoblastic leukaemia
  • Gene rearrangement
  • Minimal residual disease
  • Molecular biology
  • T cell receptor

ASJC Scopus subject areas

  • Hematology

Cite this

@article{46991d14bc4f4b09846727ed5f856ac8,
title = "Detection of T cell receptor delta gene rearrangement in childhood B and T lineage acute lymphoblastic leukaemia by southern blot and PCR: Technical comparison of two methods of analysis",
abstract = "Molecular analysis of antigen receptor genes (Ig and TCR) has been useful for clonal studies in acute lymphoblastic leukaemia (ALL) patients. Rearrangements of these genes can be used to track the persistence of the leukaemic clone during the therapy. The purpose of our study was to analyse the percentage and the pattern of the rearrangements at the TCR D locus in a series of ALL patients, comparing the results obtained by Southern blot and PCR. Genomic DNA was extracted from mononuclear BM cells of 40 paediatric ALL cases, digested with different restriction enzymes and hybridized to TCRDJ1 probe to study the TCR delta locus. Amplification of the rearranged TCR delta genes was performed by PCR to define the gene segments involved. The junctional region was deduced from the sequence to obtain patient-specific primers. Among the 31 B lineage ALL samples, one or two TCR δ alleles proved to be rearranged in 53{\%} of cases. Two different types of rearrangements were chiefly detected: Vδ2Dδ3 and Dδ2Dδ3. In T-ALL patients, the predominant rearrangement involved the Vδ1 and the Jδ1 gene segments.",
keywords = "Acute lymphoblastic leukaemia, Gene rearrangement, Minimal residual disease, Molecular biology, T cell receptor",
author = "A. Valetto and {Di Martino}, D. and F. Scuderi and T. Lanza and M. Lanciotti and F. Bottini and G. Dini",
year = "2000",
doi = "10.1046/j.1365-2257.2000.00317.x",
language = "English",
volume = "22",
pages = "263--269",
journal = "Clinical and Laboratory Haematology",
issn = "0141-9854",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Detection of T cell receptor delta gene rearrangement in childhood B and T lineage acute lymphoblastic leukaemia by southern blot and PCR

T2 - Technical comparison of two methods of analysis

AU - Valetto, A.

AU - Di Martino, D.

AU - Scuderi, F.

AU - Lanza, T.

AU - Lanciotti, M.

AU - Bottini, F.

AU - Dini, G.

PY - 2000

Y1 - 2000

N2 - Molecular analysis of antigen receptor genes (Ig and TCR) has been useful for clonal studies in acute lymphoblastic leukaemia (ALL) patients. Rearrangements of these genes can be used to track the persistence of the leukaemic clone during the therapy. The purpose of our study was to analyse the percentage and the pattern of the rearrangements at the TCR D locus in a series of ALL patients, comparing the results obtained by Southern blot and PCR. Genomic DNA was extracted from mononuclear BM cells of 40 paediatric ALL cases, digested with different restriction enzymes and hybridized to TCRDJ1 probe to study the TCR delta locus. Amplification of the rearranged TCR delta genes was performed by PCR to define the gene segments involved. The junctional region was deduced from the sequence to obtain patient-specific primers. Among the 31 B lineage ALL samples, one or two TCR δ alleles proved to be rearranged in 53% of cases. Two different types of rearrangements were chiefly detected: Vδ2Dδ3 and Dδ2Dδ3. In T-ALL patients, the predominant rearrangement involved the Vδ1 and the Jδ1 gene segments.

AB - Molecular analysis of antigen receptor genes (Ig and TCR) has been useful for clonal studies in acute lymphoblastic leukaemia (ALL) patients. Rearrangements of these genes can be used to track the persistence of the leukaemic clone during the therapy. The purpose of our study was to analyse the percentage and the pattern of the rearrangements at the TCR D locus in a series of ALL patients, comparing the results obtained by Southern blot and PCR. Genomic DNA was extracted from mononuclear BM cells of 40 paediatric ALL cases, digested with different restriction enzymes and hybridized to TCRDJ1 probe to study the TCR delta locus. Amplification of the rearranged TCR delta genes was performed by PCR to define the gene segments involved. The junctional region was deduced from the sequence to obtain patient-specific primers. Among the 31 B lineage ALL samples, one or two TCR δ alleles proved to be rearranged in 53% of cases. Two different types of rearrangements were chiefly detected: Vδ2Dδ3 and Dδ2Dδ3. In T-ALL patients, the predominant rearrangement involved the Vδ1 and the Jδ1 gene segments.

KW - Acute lymphoblastic leukaemia

KW - Gene rearrangement

KW - Minimal residual disease

KW - Molecular biology

KW - T cell receptor

UR - http://www.scopus.com/inward/record.url?scp=0034526573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034526573&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2257.2000.00317.x

DO - 10.1046/j.1365-2257.2000.00317.x

M3 - Article

C2 - 11122266

AN - SCOPUS:0034526573

VL - 22

SP - 263

EP - 269

JO - Clinical and Laboratory Haematology

JF - Clinical and Laboratory Haematology

SN - 0141-9854

IS - 5

ER -