Abstract
HBeAg/anti-HBe seroconversion is associated, in some patients affected by type B chronic active hepatitis (CAH), with the occurrence of HBV pre-core mutants characterized by a common G-A change at codon 83. Since this mutation has important clinical correlations, we tried to develop a fast and reliable PCR test based on the amplification refractory mutation system (ARMS) technique, which has been successfully used to identify point mutations associated with genetic diseases. Following this approach, we analysed HBV particles isolated from 7 patients with anti-HBe CAH and previously characterized by DNA sequencing. Sera containing only wild type or mutant HBV DNA or a combination of both showed a discrete amplification product only in the presence of the specific wild type or mutant upstream primer. These results confirm the efficacy of the ARMS technique in detecting in a rapid and specific fashion the most common and clinically relevant HBV pre-core mutation.
| Original language | English |
|---|---|
| Pages (from-to) | 307-309 |
| Number of pages | 3 |
| Journal | Research in Virology |
| Volume | 144 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1993 |
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Keywords
- HBV, PCR, ARMS, Pre-core, mutation
- Sequence, HBeAg, anti-HBe, Technique
ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Detection of the hepatitis B virus major pre-core mutation by the amplification refractory mutation system technique. / Gramegna, M.; Lampertico, P.; Lobbiani, A.; Colucci, G.
In: Research in Virology, Vol. 144, No. C, 1993, p. 307-309.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of the hepatitis B virus major pre-core mutation by the amplification refractory mutation system technique
AU - Gramegna, M.
AU - Lampertico, P.
AU - Lobbiani, A.
AU - Colucci, G.
PY - 1993
Y1 - 1993
N2 - HBeAg/anti-HBe seroconversion is associated, in some patients affected by type B chronic active hepatitis (CAH), with the occurrence of HBV pre-core mutants characterized by a common G-A change at codon 83. Since this mutation has important clinical correlations, we tried to develop a fast and reliable PCR test based on the amplification refractory mutation system (ARMS) technique, which has been successfully used to identify point mutations associated with genetic diseases. Following this approach, we analysed HBV particles isolated from 7 patients with anti-HBe CAH and previously characterized by DNA sequencing. Sera containing only wild type or mutant HBV DNA or a combination of both showed a discrete amplification product only in the presence of the specific wild type or mutant upstream primer. These results confirm the efficacy of the ARMS technique in detecting in a rapid and specific fashion the most common and clinically relevant HBV pre-core mutation.
AB - HBeAg/anti-HBe seroconversion is associated, in some patients affected by type B chronic active hepatitis (CAH), with the occurrence of HBV pre-core mutants characterized by a common G-A change at codon 83. Since this mutation has important clinical correlations, we tried to develop a fast and reliable PCR test based on the amplification refractory mutation system (ARMS) technique, which has been successfully used to identify point mutations associated with genetic diseases. Following this approach, we analysed HBV particles isolated from 7 patients with anti-HBe CAH and previously characterized by DNA sequencing. Sera containing only wild type or mutant HBV DNA or a combination of both showed a discrete amplification product only in the presence of the specific wild type or mutant upstream primer. These results confirm the efficacy of the ARMS technique in detecting in a rapid and specific fashion the most common and clinically relevant HBV pre-core mutation.
KW - HBV, PCR, ARMS, Pre-core, mutation
KW - Sequence, HBeAg, anti-HBe, Technique
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UR - http://www.scopus.com/inward/citedby.url?scp=0027267540&partnerID=8YFLogxK
U2 - 10.1016/S0923-2516(06)80046-4
DO - 10.1016/S0923-2516(06)80046-4
M3 - Article
C2 - 8210714
AN - SCOPUS:0027267540
VL - 144
SP - 307
EP - 309
JO - Research in Virology
JF - Research in Virology
SN - 0923-2516
IS - C
ER -