Detection of the incX3 plasmid carrying blaKPC-3 in a Serratia marcescens strain isolated from a kidney–liver transplanted patient

Floriana Gona, Carla Caio, Gioacchin Iannolo, Francesco Monaco, Giuseppina Di Mento, Nicola Cuscino, Ignazio Fontana, Giovanna Panarello, Gaetano Maugeri, Maria Lina Mezzatesta, Stefania Stefani, Pier Giulio Conaldi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dissemination of resistance to carbapenems among Enterobacteriaceae through plasmids is an increasingly important concern in health care worldwide. Here we report the first description of an IncX3 plasmid carrying the blaKPC-3 gene in a strain of Serratia marcescens isolated from a kidney-liver transplanted patient at the transplantation centre ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione, Palermo, Italy). To localize the transposable element containing the resistance-associated gene Next-Generation Sequencing of the bacterial DNA was performed. S. marcescens was positive for blaKPC-3 and blaSHV-11 genes. The molecular analysis demonstrated that the blaKPC-3 gene of this bacterial strain was located in one copy of the Tn-3-like element Tn4401-a carried in a plasmid that is 53 392 bp in size and showed the typical IncX3 scaffold. Our data demonstrated the presence of a new blaKPC-3 harbouring the IncX3 plasmid in S. marcescens. The possible dissemination among Enterobacteriaceae of this type of plasmid should be monitored and evaluated in terms of clinical risk.

Original languageEnglish
Article number000592
Pages (from-to)1454-1456
Number of pages3
JournalJournal of Medical Microbiology
Volume66
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Serratia marcescens
Plasmids
Enterobacteriaceae
Genes
Bacterial Genes
Bacterial DNA
Carbapenems
DNA Transposable Elements
Italy
Transplantation
Delivery of Health Care
Kidney
Liver

Keywords

  • IncX
  • KPC
  • Serratia marcescens
  • Transplantation

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Detection of the incX3 plasmid carrying blaKPC-3 in a Serratia marcescens strain isolated from a kidney–liver transplanted patient. / Gona, Floriana; Caio, Carla; Iannolo, Gioacchin; Monaco, Francesco; Di Mento, Giuseppina; Cuscino, Nicola; Fontana, Ignazio; Panarello, Giovanna; Maugeri, Gaetano; Mezzatesta, Maria Lina; Stefani, Stefania; Conaldi, Pier Giulio.

In: Journal of Medical Microbiology, Vol. 66, No. 10, 000592, 01.10.2017, p. 1454-1456.

Research output: Contribution to journalArticle

Gona, Floriana ; Caio, Carla ; Iannolo, Gioacchin ; Monaco, Francesco ; Di Mento, Giuseppina ; Cuscino, Nicola ; Fontana, Ignazio ; Panarello, Giovanna ; Maugeri, Gaetano ; Mezzatesta, Maria Lina ; Stefani, Stefania ; Conaldi, Pier Giulio. / Detection of the incX3 plasmid carrying blaKPC-3 in a Serratia marcescens strain isolated from a kidney–liver transplanted patient. In: Journal of Medical Microbiology. 2017 ; Vol. 66, No. 10. pp. 1454-1456.
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