Detection of tissue factor antigen and coagulation activity in coronary artery thrombi isolated from patients with ST-segment elevation acute myocardial infarction

Tullio Palmerini, Luciana Tomasi, Chiara Barozzi, Diego Della Riva, Andrea Mariani, Nevio Taglieri, Ornella Leone, Claudio Ceccarelli, Stefano De Servi, Angelo Branzi, Philippe Genereux, Gregg W. Stone, Jasimuddin Ahamed

Research output: Contribution to journalArticle

Abstract

Introduction: Although ruptured atherosclerotic plaques have been extensively analyzed, the composition of thrombi causing arterial occlusion in patients with ST-segment elevation acute myocardial infarction has been less thoroughly investigated. We sought to investigate whether coagulant active tissue factor can be retrieved in thrombi of patients with STEMI undergoing primary percutaneous coronary intervention. Methods: Nineteen patients with ST-segment elevation acute myocardial infarction referred for primary percutaneous coronary intervention were enrolled in this study. Coronary thrombi aspirated from coronary arteries were routinely processed for paraffin embedding and histological evaluation (4 patients) or immediately snap frozen for evaluation of tissue factor activity using a modified aPTT test (15 patients). Immunoprecipitation followed by immunoblotting was also performed in 12 patients. Results: Thrombi aspirated from coronary arteries showed large and irregular areas of tissue factor staining within platelet aggregates, and in close contact with inflammatory cells. Some platelet aggregates stained positive for tissue factor, whereas others did not. Monocytes consistently stained strongly for tissue factor, neutrophils had a more variable and irregular tissue factor staining, and red blood cells did not demonstrate staining for tissue factor. Median clotting time of plasma samples containing homogenized thrombi incubated with a monoclonal antibody that specifically inhibits tissue factor-mediated coagulation activity (mAb 5G9) were significantly longer than their respective controls (88.9 seconds versus 76.5 seconds, respectively; p

Original languageEnglish
Article numbere81501
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 11 2013

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Palmerini, T., Tomasi, L., Barozzi, C., Della Riva, D., Mariani, A., Taglieri, N., Leone, O., Ceccarelli, C., De Servi, S., Branzi, A., Genereux, P., Stone, G. W., & Ahamed, J. (2013). Detection of tissue factor antigen and coagulation activity in coronary artery thrombi isolated from patients with ST-segment elevation acute myocardial infarction. PLoS One, 8(12), [e81501]. https://doi.org/10.1371/journal.pone.0081501