Detection of two functional polymorphisms in the promoter region of the IL-18 gene by single-tube allele specific PCR and melting temperature analysis

Michele Boniotto, Ludovica Segat, Michele Milanese, Sergio Crovella

Research output: Contribution to journalArticlepeer-review

Abstract

IL-18 is an important cytokine in both innate and acquired immunity. IL-18 promoter polymorphisms have been investigated in several autoimmune and infectious diseases. Here we present two assays for a medium to a high throughput genotyping of C-607A and G-137C IL-18 SNPs. Using these protocols we have been able to rapidly genotype a cohort of 131 healthy Italian individuals. The assays, based on a multiplex allele-specific PCR and the melting temperature analysis of the PCR product, are rapid, easily automated, inexpensive and well suited to case-control association studies.

Original languageEnglish
Pages (from-to)184-188
Number of pages5
JournalJournal of Immunological Methods
Volume304
Issue number1-2
DOIs
Publication statusPublished - Sep 2005

Keywords

  • Association studies
  • IL-18
  • Melting analysis
  • SNP genotyping
  • SYBR green I

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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